Establishment of cell culture model and humanized mouse model of chronic hepatitis B virus infection

Approximately 257 million people worldwide have been infected with hepatitis B virus (HBV), and HBV infection can cause chronic hepatitis, cirrhosis, and even liver cancer. The lack of suitable and effective infection models has greatly limited research in HBV-related fields for a long time, and it...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology spectrum 2024-01, Vol.12 (1), p.e0274523-e0274523
Main Authors: Tian, Renyun, Yang, Di, Xu, Biaoming, Deng, Rilin, Xue, Binbin, Wang, Luoling, Li, Huiyi, Liu, Qian, Wang, Xiaohong, Tang, Songqing, Wan, Mengyu, Pei, Hua, Zhu, Haizhen
Format: Article
Language:English
Subjects:
animal model
Animals
cell culture model
Cell Culture Techniques
Disease Models, Animal
HBV persistence
Hepatitis B
Hepatitis B virus - genetics
Hepatitis B, Chronic
Host-Microbial Interactions
Humans
kinetics of antigen
Mice
Research Article
virion secretion
Virus Replication
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Approximately 257 million people worldwide have been infected with hepatitis B virus (HBV), and HBV infection can cause chronic hepatitis, cirrhosis, and even liver cancer. The lack of suitable and effective infection models has greatly limited research in HBV-related fields for a long time, and it is still not possible to discover a method to completely and effectively remove the HBV genome. We have constructed a hepatocellular carcinoma cell line, HLCZ01, that can support the complete life cycle of HBV. This model can mimic the long-term stable infection of HBV in the natural state and can replace primary human hepatocytes for the development of human liver chimeric mice. This model will be a powerful tool for research in the field of HBV.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.02745-23