Fucoidan Suppresses Mitochondrial Dysfunction and Cell Death against 1-Methyl-4-Phenylpyridinum-Induced Neuronal Cytotoxicity via Regulation of PGC-1α Expression

• Published in: Marine drugs 2019-09, Vol.17 (9), p.518
• Main Authors: Han, Yong-Seok, Lee, Jun Hee, Lee, Sang Hun
• Format: Article
• Language: English
• Subjects: 1-Methyl-4-phenylpyridinium - pharmacology; Adenosine kinase; Adenosine monophosphate; Adenylate Kinase - metabolism; Algae; AMP; AMP-activated protein kinase; Apoptosis; Biosynthesis; Cell death; Cell Death - drug effects; Cell Line, Tumor; Cells; Chemical compounds; Chronic illnesses; Cytotoxicity; Dopamine receptors; Dopaminergic Neurons - drug effects; Fucoidan; Humans; Kinases; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondrial Diseases - drug therapy; Mitochondrial Diseases - metabolism; Mitochondrial DNA; mitochondrial dysfunction; Molecular weight; Movement disorders; MPP; Neurodegenerative diseases; Neurons; Neurons - drug effects; Neurons - metabolism; Neurotoxins; Organelles; Oxidative stress; Parkinson Disease - drug therapy; Parkinson Disease - metabolism; Parkinson's disease; Pathogenesis; patients; peroxisome proliferator-activated receptor gamma; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; PGC-1α; Pharmacology; Phosphorylation; Polysaccharides - pharmacology; Protein kinase; Pyridinium; Senescence; Stem cells; therapeutics; Toxicity; Toxins
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md17090518

Mitochondria are considered to be the powerhouses of cells. They are the most commonly damaged organelles within dopaminergic neurons in patients with Parkinson's disease (PD). Despite the importance of protecting neuronal mitochondria in PD patients, the detailed mechanisms underlying mitochondrial dysfunction during pathogenesis and pathophysiological progression of PD have not yet been elucidated. We investigated the protective action of fucoidan against the detrimental action of 1-methyl-4-phenyl-pyridinium (MPP ), a neurotoxin used to model PD, in the mitochondria of SH-SY5Y neural cells. Fucoidan increased the expression of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) and protected the cells from MPP -induced apoptosis by upregulating the 5' adenosine monophosphate-activated protein kinase (AMPK)-PGC-1α axis. These effects were blocked by the silencing of the PGC-1α axis. These results indicated that fucoidan protects SH-SY5Y cells from mitochondrial dysfunction and cell death caused by MPP treatment, via the AMPK-PGC-1α axis. These findings also suggest that fucoidan could potentially be used as a therapeutic agent for PD.