Suppression of cdc13-2-associated senescence by pif1-m2 requires Ku-mediated telomerase recruitment

In Saccharomyces cerevisiae, recruitment of telomerase to telomeres requires an interaction between Cdc13, which binds single-stranded telomeric DNA, and the Est1 subunit of telomerase. A second pathway involving an interaction between the yKu complex and telomerase RNA (TLC1) contributes to telomer...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2022-01, Vol.12 (1)
Main Authors: Fekete-Szücs, Enikő, Rosas Bringas, Fernando R, Stinus, Sonia, Chang, Michael
Format: Article
Language:English
Subjects:
DNA Helicases - genetics
DNA Helicases - metabolism
DNA-Binding Proteins
Investigation
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Telomerase - genetics
Telomerase - metabolism
Telomere - genetics
Telomere - metabolism
Telomere-Binding Proteins - genetics
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Summary:In Saccharomyces cerevisiae, recruitment of telomerase to telomeres requires an interaction between Cdc13, which binds single-stranded telomeric DNA, and the Est1 subunit of telomerase. A second pathway involving an interaction between the yKu complex and telomerase RNA (TLC1) contributes to telomerase recruitment but cannot sufficiently recruit telomerase on its own to prevent replicative senescence when the primary Cdc13-Est1 pathway is abolished—for example, in the cdc13-2 mutant. In this study, we find that mutation of PIF1, which encodes a helicase that inhibits telomerase, suppresses the replicative senescence of cdc13-2 by increasing reliance on the yKu-TLC1 pathway for telomerase recruitment. Our findings reveal new insight into telomerase-mediated telomere maintenance.
ISSN:2160-1836
2160-1836
DOI:10.1093/g3journal/jkab360