Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

• Published in: International journal of molecular sciences 2022-12, Vol.23 (23), p.15100
• Main Authors: Vasyutkina, Elena A, Yugay, Yulia A, Grigorchuk, Valeria P, Grishchenko, Olga V, Sorokina, Maria R, Yaroshenko, Yulia L, Kudinova, Olesya D, Stepochkina, Varvara D, Bulgakov, Victor P, Shkryl, Yury N
• Format: Article
• Language: English
• Subjects: Abiotic stress; Accumulation; Acids; Agrobacterium rhizogenes; Antioxidants; Biomass; Biosynthesis; C gene; Caffeoylquinic acid; Cell culture; Cell Culture Techniques; Cold; Crops; Deoxyribonucleic acid; Developing countries; DNA; DNA - metabolism; Food; Gene expression; Gene Expression Regulation, Plant; Ipomoea batatas; Ipomoea batatas - genetics; Ipomoea batatas - metabolism; LDCs; Light; Metabolism; Metabolites; Methyl jasmonate; Molecular modelling; naturally transgenic plants; Nucleotide sequence; Open reading frames; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; polyphenols; Potatoes; rol genes; Rooting; Salicylic acid; Salicylic Acid - metabolism; Salicylic Acid - pharmacology; Secondary Metabolism; sweet potato; Sweet potatoes; T-DNA; Transgenes; Transgenic plants; Ultraviolet radiation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232315100

is a vital root crop and a source of caffeoylquinic acid derivatives (CQAs) with potential health-promoting benefits. As a naturally transgenic plant, contains cellular T-DNA (cT-DNA) sequence homologs of the open reading frame (ORF)14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n of unknown function. This study aimed to evaluate the effect of abiotic stresses (temperature, ultraviolet, and light) and chemical elicitors (methyl jasmonate, salicylic acid, and sodium nitroprusside) on the biosynthesis of CQAs and cT-DNA gene expression in cell culture as a model system. Among all the applied treatments, ultraviolet irradiation, methyl jasmonate, and salicylic acid caused the maximal accumulation of secondary compounds. We also discovered that cT-DNA genes were not expressed in cell culture, and the studied conditions weakly affected their transcriptional levels. However, the gene expressed under the strong 35S CaMV promoter increased the CQAs content by 1.5-1.9-fold. Overall, our results show that cT-DNA-encoded transgenes are not involved in stress- and chemical elicitor-induced CQAs accumulation in cell cultures of . Nevertheless, overaccumulation of RolB/RolC transcripts potentiates the secondary metabolism of sweet potatoes through a currently unknown mechanism. Our study provides new insights into the molecular mechanisms linked with CQAs biosynthesis in cell culture of naturally transgenic food crops, i.e., sweet potato.