Effect of Disulfiram on the Reproductive Capacity of Female Mice

In the process of assisted reproduction, the high-oxygen in vitro environment can easily cause oxidative damage to oocytes. Disulfiram (DSF) can play an anti-oxidant or pro-oxidant role in different cells, and the effect of DSF on oocytes remains unclear. Moreover, it remains unclear whether the use...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-01, Vol.24 (3), p.2371
Main Authors: Teng, Mingming, Luo, Yuan, Wang, Chan, Lei, Anmin
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Alcohol
Angiogenesis
Animals
Antioxidants
Antioxidants - metabolism
Antioxidants - pharmacology
Cell Line, Tumor
Disulfiram
Disulfiram - pharmacology
Embryos
Female
female mice
Fetuses
Follicle-stimulating hormone
Gametocytes
Gene expression
Genes
Hormones
In vitro fertilization
Kinases
MAP kinase
Mice
Oocytes
Ovulation
Oxidants
Oxidation
Oxidative Stress
Oxidizing agents
Pregnancy
Proteins
Reactive Oxygen Species - metabolism
Reproduction
reproductive capacity
TOR protein
Uterus
Vagina
Vascular endothelial growth factor
Vascularization
Womens health
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Summary:In the process of assisted reproduction, the high-oxygen in vitro environment can easily cause oxidative damage to oocytes. Disulfiram (DSF) can play an anti-oxidant or pro-oxidant role in different cells, and the effect of DSF on oocytes remains unclear. Moreover, it remains unclear whether the use of DSF in the early stages of pregnancy has a negative impact on the fetus. In this study, we found that DSF increased serum FSH levels and increased the ovulation rate in mice. Moreover, DSF enhanced the antioxidant capacity of oocytes and contributed to the success rate of in vitro fertilization. Moreover, the use of DSF in early pregnancy in mice increased the uterine horn volume and the degree of vascularization, which contributed to a successful pregnancy. In addition, it was found that DSF regulated the mRNA expression of angiogenesis-related genes ( ), follicular development-related genes ( , and ), ovulation-related genes ( , and ) and antioxidant-related genes ( and ). These results indicate that DSF is helpful for increasing the antioxidant capacity of oocytes and the ovulation rate. In early pregnancy in mice, DSF promotes pregnancy by increasing the degree and volume of uterine vascularization.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032371