Target Recognition– and HCR Amplification–Induced In Situ Electrochemical Signal Probe Synthesis Strategy for Trace ctDNA Analysis

An electrochemical-DNA (E-DNA) sensor was constructed by using DNA metallization to produce an electrochemical signal reporter in situ and hybridization chain reaction (HCR) as signal amplification strategy. The cyclic voltammetry (CV) technique was used to characterize the electrochemical solid-sta...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-11, Vol.12 (11), p.989
Main Authors: Cai, Aiting, Yang, Luxia, Kang, Xiaoxia, Liu, Jinxia, Wang, Feng, Ji, Haiwei, Wang, Qi, Wu, Mingmin, Li, Guo, Zhou, Xiaobo, Qin, Yuling, Wu, Li
Format: Article
Language:English
Subjects:
Amplification
Analysis
Biosensors
Blood
Cancer
ctDNA
Deoxyribonucleic acid
DNA
DNA metallization
DNA sequencing
E-DNA sensor
Electrochemical analysis
Electrochemistry
Electrodes
Exo Ι
Feasibility studies
HCR
Health aspects
Hybridization
Medical examination
Metallizing
Methods
Nucleotide sequencing
Selectivity
Sensors
Signal processing
Silver
Silver chloride
Spectrum analysis
Target recognition
Voltammetry
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Summary:An electrochemical-DNA (E-DNA) sensor was constructed by using DNA metallization to produce an electrochemical signal reporter in situ and hybridization chain reaction (HCR) as signal amplification strategy. The cyclic voltammetry (CV) technique was used to characterize the electrochemical solid-state Ag/AgCl process. Moreover, the enzyme cleavage technique was introduced to reduce background signals and further improve recognition accuracy. On the basis of these techniques, the as-prepared E-DNA sensor exhibited superior sensing performance for trace ctDNA analysis with a detection range of 0.5 fM to 10 pM and a detection limit of 7 aM. The proposed E-DNA sensor also displayed excellent selectivity, satisfied repeatability and stability, and had good recovery, all of which supports its potential applications for future clinical sample analysis.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12110989