Lactobacillus oris improves non-alcoholic fatty liver in mice and inhibits endogenous cholesterol biosynthesis

• Published in: Scientific reports 2023-08, Vol.13 (1), p.12946-12946, Article 12946
• Main Authors: Zhao, Yiming, Li, Congyong, Luan, Zhe, Chen, Jun, Wang, Cong, Jing, Yujia, Qi, Shirui, Zhao, Zhizhuang, Zhang, Hanwen, Wu, Junling, Chen, Yi, Li, Zhuanyu, Zhao, Bowen, Wang, Shufang, Yang, Yunsheng, Sun, Gang
• Format: Article
• Language: English
• Subjects: 631/326; 692/308; 692/4020; Aging; Animal models; Bile acids; Biosynthesis; Cell culture; Centenarians; Cholesterol; Fatty liver; Feces; Geriatrics; Humanities and Social Sciences; Lactobacillus; Lipids; multidisciplinary; Oldest old people; Probiotics; Reabsorption; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-38530-x

We previously confirmed that a strain of Lactobacillus oris isolated from the fecal samples of healthy Hainan centenarian having potent lipid-lowering ability in HepG2 cells; and this study was to investigate the effect of the stain on non-alcoholic fatty liver in mice in vivio. The Lactobacillus oris strain isolated from Hainan centenarian fecal samples were frozen stored in our laboratory. Thirty ob/ob mice (10 in each group) were orally gavaged with Lactobacillus oris (Lactobacillus, 5 × 10 9  cfu), mixed probiotics (Mixed, 5 × 10 9  cfu, a mixture with known lipid-lowering ability), or culture medium (Control) respectively. Lactobacillus oris isolated from fecal samples of Hainan centenarians showed significantly in vivo lipid lowering ability compared with the controls, and the ability was comparable with mixed probiotics strains in mice The possible mechanisms of lipid-lowering of probiotics and Lactobacillus oris may be associated with HMGR inhibition to suppress the synthesis of endogenous cholesterol; bile acids reabsorption, and intestinal FXR-FGF15 signaling pathways promoting the cholesterol conversion into bile acids secretion.