Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy

This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against , , , , and by t...

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Published in:Journal of functional biomaterials 2022-12, Vol.13 (4), p.305
Main Authors: Braga, Gabriela Pacheco de Almeida, Caiaffa, Karina Sampaio, Pereira, Jesse Augusto, Santos, Vanessa Rodrigues Dos, Souza, Amanda Caselato Andolfatto, Ribeiro, Lucas da Silva, Camargo, Emerson Rodrigues, Prakki, Anuradha, Duque, Cristiane
Format: Article
Language:English
Subjects:
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antioxidants
Bacteria
Biocompatibility
Biofilms
Calcium compounds
Calcium hydroxide
Cancer
Chemical properties
Chlorhexidine
Confocal microscopy
Cytotoxicity
Endodontics
Ethylenediaminetetraacetic acid
Fibroblasts
Flavonoids
hydrogel
Hydrogels
Hydroxides
Infections
Isoflavones
Laboratories
Microbiology
N-vinylcaprolactam
Rheological properties
Rheology
Root canals
Rutin
Scanning electron microscopy
Scanning microscopy
Slaked lime
Toxicity
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Summary:This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against , , , , and by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against , and . SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes.
ISSN:2079-4983
2079-4983
DOI:10.3390/jfb13040305