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Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy
This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against , , , , and by t...
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| Published in: | Journal of functional biomaterials 2022-12, Vol.13 (4), p.305 |
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| creator | Braga, Gabriela Pacheco de Almeida Caiaffa, Karina Sampaio Pereira, Jesse Augusto Santos, Vanessa Rodrigues Dos Souza, Amanda Caselato Andolfatto Ribeiro, Lucas da Silva Camargo, Emerson Rodrigues Prakki, Anuradha Duque, Cristiane |
| description | This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against
,
,
,
, and
by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against
,
and
. SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes. |
| doi_str_mv | 10.3390/jfb13040305 |
| format | article |
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,
,
,
, and
by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against
,
and
. SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes.</description><identifier>ISSN: 2079-4983</identifier><identifier>EISSN: 2079-4983</identifier><identifier>DOI: 10.3390/jfb13040305</identifier><identifier>PMID: 36547565</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial activity ; Antimicrobial agents ; Antioxidants ; Bacteria ; Biocompatibility ; Biofilms ; Calcium compounds ; Calcium hydroxide ; Cancer ; Chemical properties ; Chlorhexidine ; Confocal microscopy ; Cytotoxicity ; Endodontics ; Ethylenediaminetetraacetic acid ; Fibroblasts ; Flavonoids ; hydrogel ; Hydrogels ; Hydroxides ; Infections ; Isoflavones ; Laboratories ; Microbiology ; N-vinylcaprolactam ; Rheological properties ; Rheology ; Root canals ; Rutin ; Scanning electron microscopy ; Scanning microscopy ; Slaked lime ; Toxicity</subject><ispartof>Journal of functional biomaterials, 2022-12, Vol.13 (4), p.305</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-8ddf5f3f3f4b93e5a05f285ce9a4051df67ca4dd132ecf9900f708f83c5f1c2f3</citedby><cites>FETCH-LOGICAL-c542t-8ddf5f3f3f4b93e5a05f285ce9a4051df67ca4dd132ecf9900f708f83c5f1c2f3</cites><orcidid>0000-0002-2575-279X ; 0000-0001-8725-2136 ; 0000-0001-6314-0076 ; 0000-0002-8969-9043</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2756723934/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2756723934?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36547565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Braga, Gabriela Pacheco de Almeida</creatorcontrib><creatorcontrib>Caiaffa, Karina Sampaio</creatorcontrib><creatorcontrib>Pereira, Jesse Augusto</creatorcontrib><creatorcontrib>Santos, Vanessa Rodrigues Dos</creatorcontrib><creatorcontrib>Souza, Amanda Caselato Andolfatto</creatorcontrib><creatorcontrib>Ribeiro, Lucas da Silva</creatorcontrib><creatorcontrib>Camargo, Emerson Rodrigues</creatorcontrib><creatorcontrib>Prakki, Anuradha</creatorcontrib><creatorcontrib>Duque, Cristiane</creatorcontrib><title>Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy</title><title>Journal of functional biomaterials</title><addtitle>J Funct Biomater</addtitle><description>This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against
,
,
,
, and
by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against
,
and
. SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes.</description><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Antioxidants</subject><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Biofilms</subject><subject>Calcium compounds</subject><subject>Calcium hydroxide</subject><subject>Cancer</subject><subject>Chemical properties</subject><subject>Chlorhexidine</subject><subject>Confocal microscopy</subject><subject>Cytotoxicity</subject><subject>Endodontics</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fibroblasts</subject><subject>Flavonoids</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>Hydroxides</subject><subject>Infections</subject><subject>Isoflavones</subject><subject>Laboratories</subject><subject>Microbiology</subject><subject>N-vinylcaprolactam</subject><subject>Rheological properties</subject><subject>Rheology</subject><subject>Root canals</subject><subject>Rutin</subject><subject>Scanning electron microscopy</subject><subject>Scanning microscopy</subject><subject>Slaked lime</subject><subject>Toxicity</subject><issn>2079-4983</issn><issn>2079-4983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptks1vEzEQxVcIRKvSE3dkiQtSlWKv7fX6glRFLY2UQg-Fq-X1x9bRxhPsTUXu_OE4TSkJwnOwZb_3k2b8quotweeUSvxx4TtCMcMU8xfVcY2FnDDZ0pd756PqNOcFLqvBbU3Y6-qINpwJ3vDj6tdNMAm6AAP0wegB3SZYuTQGl5GOFk03I4zwM5gwbhB4dPvl-3SOrjc2Qe-GjKYQRx1iiD26GvQDRAi2ODOaxTFpo2NB3jhb0GOAiDwkdBkt2GILBt3du6RXmzfVK6-H7E6f9pPq29Xl3fR6Mv_6eTa9mE8MZ_U4aa313NNSrJPUcY25r1tunNQMc2J9I4xm1hJaO-OlxNgL3PqWGu6JqT09qWY7rgW9UKsUljptFOigHi8g9UqX1s3glOa6o0x2zGjCaEM72xJOjC-TdlwYW1ifdqzVuls6a9y23-EAevgSw73q4UHJllNa1wXw4QmQ4Mfa5VEtQzZuGHR0sM6qFlxgzqXARfr-H-kC1qmM9lHViJpKyv6qel0aCNHD9ge2UHUhOClJIGKrOv-PqpR1y2AgOh_K_YHhbGcoOck5Of_cI8Fqm0G1l8Gifrc_lmftn8TR347p2FI</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Braga, Gabriela Pacheco de 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Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy</title><author>Braga, Gabriela Pacheco de Almeida ; Caiaffa, Karina Sampaio ; Pereira, Jesse Augusto ; Santos, Vanessa Rodrigues Dos ; Souza, Amanda Caselato Andolfatto ; Ribeiro, Lucas da Silva ; Camargo, Emerson Rodrigues ; Prakki, Anuradha ; Duque, Cristiane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-8ddf5f3f3f4b93e5a05f285ce9a4051df67ca4dd132ecf9900f708f83c5f1c2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Antioxidants</topic><topic>Bacteria</topic><topic>Biocompatibility</topic><topic>Biofilms</topic><topic>Calcium compounds</topic><topic>Calcium hydroxide</topic><topic>Cancer</topic><topic>Chemical properties</topic><topic>Chlorhexidine</topic><topic>Confocal microscopy</topic><topic>Cytotoxicity</topic><topic>Endodontics</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fibroblasts</topic><topic>Flavonoids</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>Hydroxides</topic><topic>Infections</topic><topic>Isoflavones</topic><topic>Laboratories</topic><topic>Microbiology</topic><topic>N-vinylcaprolactam</topic><topic>Rheological properties</topic><topic>Rheology</topic><topic>Root canals</topic><topic>Rutin</topic><topic>Scanning electron microscopy</topic><topic>Scanning microscopy</topic><topic>Slaked lime</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Braga, Gabriela Pacheco de Almeida</creatorcontrib><creatorcontrib>Caiaffa, Karina Sampaio</creatorcontrib><creatorcontrib>Pereira, Jesse Augusto</creatorcontrib><creatorcontrib>Santos, 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PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy</atitle><jtitle>Journal of functional biomaterials</jtitle><addtitle>J Funct Biomater</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>13</volume><issue>4</issue><spage>305</spage><pages>305-</pages><issn>2079-4983</issn><eissn>2079-4983</eissn><abstract>This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against
,
,
,
, and
by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against
,
and
. SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36547565</pmid><doi>10.3390/jfb13040305</doi><orcidid>https://orcid.org/0000-0002-2575-279X</orcidid><orcidid>https://orcid.org/0000-0001-8725-2136</orcidid><orcidid>https://orcid.org/0000-0001-6314-0076</orcidid><orcidid>https://orcid.org/0000-0002-8969-9043</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of functional biomaterials, 2022-12, Vol.13 (4), p.305 |
| issn | 2079-4983 2079-4983 |
| language | eng |
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| source | Open Access: PubMed Central; Publicly Available Content Database (Proquest) (PQ_SDU_P3) |
| subjects | Antibiotics Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Antioxidants Bacteria Biocompatibility Biofilms Calcium compounds Calcium hydroxide Cancer Chemical properties Chlorhexidine Confocal microscopy Cytotoxicity Endodontics Ethylenediaminetetraacetic acid Fibroblasts Flavonoids hydrogel Hydrogels Hydroxides Infections Isoflavones Laboratories Microbiology N-vinylcaprolactam Rheological properties Rheology Root canals Rutin Scanning electron microscopy Scanning microscopy Slaked lime Toxicity |
| title | Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy |
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