Genetic characterization of feline panleukopenia virus from dogs in Vietnam reveals a unique Thr101 mutation in VP2

• Published in: PeerJ (San Francisco, CA) CA), 2020-10, Vol.8, p.e9752-e9752, Article e9752
• Main Authors: Hoang, Minh, Wu, Cheng-Nan, Lin, Chuen-Fu, Nguyen, Huong Thanh Thi, Le, Van Phan, Chiou, Ming-Tang, Lin, Chao-Nan
• Format: Article
• Language: English
• Subjects: 3D structure; Amino acids; Analysis; Binding sites; Bone marrow; Canine parvovirus type 2; Deoxyribonucleic acid; Diarrhea; DNA; Dogs; Domestic cats; Feline panleukopenia; Feline parvovirus; Gene amplification; Genetic aspects; Genotypes; Maximum likelihood method; Microbiology; Mutation; Open source software; Parvoviruses; Phylogenetics; Phylogeny; Proteins; Sequence analysis; Sequences analysis; Strains (organisms); Veterinary Medicine; Virology; Viruses; Vomiting; VP2 protein
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.9752

Canine parvovirus type 2 (CPV-2) and feline parvovirus (FPV) are known as the main causes of several serious diseases and have a severe impact on puppies and kittens, respectively. FPV and new CPV-2 variants are all able to infect cats, causing diseases indistinguishable from feline panleukopenia. However, FPV only replicates efficiently in feline cells in vitro and replicates in dogs in the thymus and bone marrow without being shed in feces. In our previous study, the genotypes of six parvoviral isolates were unable to be identified using a SimpleProbe real-time PCR assay. In the present study, we characterized previously unidentified FPV-like viruses isolated from dogs in Vietnam. The six isolates were utilized to complete VP2 gene sequencing and to conduct phylogenetic analyses. Sequence analysis of the six parvoviral strains identified the species as being similar to FPV. Phylogenetic analysis demonstrated that the complete VP2 genes of the strains are similar to those of FPV. The FPV-like strains contain a Thr101 mutation in the VP2 protein, which is different from prototype FPV strains. Our data provide evidence for the existence of changes in the charge, protein contact potential and molecular surface of the core of the receptor-binding size with an Ile101 to Thr101 mutation. This is also the first study to provide reliable evidence that FPV may be a threat to the Vietnamese dog population.