The Barley Heavy Metal Associated Isoprenylated Plant Protein HvFP1 Is Involved in a Crosstalk between the Leaf Development and Abscisic Acid-Related Drought Stress Responses

The heavy metal associated isoprenylated plant proteins (HIPPs) are characterized by at least one heavy metal associated (HMA) domain and a C-terminal isoprenylation motif. Hordeum vulgare farnesylated protein 1 (HvFP1), a barley HIPP, is upregulated during drought stress, in response to abscisic ac...

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Bibliographic Details
Published in:Plants (Basel) 2022-10, Vol.11 (21), p.2851
Main Authors: Parasyri, Athina, Barth, Olaf, Zschiesche, Wiebke, Humbeck, Klaus
Format: Article
Language:English
Subjects:
ABA
Abiotic stress
Abscisic acid
Agricultural research
Barley
Biosynthesis
Chlorophyll
Control theory
crosstalk
Development
Drought
Efficiency
Feedback loops
Gene expression
Gene sequencing
Genes
Genetic aspects
Genomes
Hardiness
Heavy metals
HIPPs
Investigations
Leaves
Negative feedback
Photomorphogenesis
Photosynthesis
Physiological aspects
Plant proteins
Plants
Plants (botany)
Proteins
Senescence
Signal transduction
Stress response
Transcriptomics
Zinc
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Summary:The heavy metal associated isoprenylated plant proteins (HIPPs) are characterized by at least one heavy metal associated (HMA) domain and a C-terminal isoprenylation motif. Hordeum vulgare farnesylated protein 1 (HvFP1), a barley HIPP, is upregulated during drought stress, in response to abscisic acid (ABA) and during leaf senescence. To investigate the role of HvFP1, two independent gain-of-function lines were generated. In a physiological level, the overexpression of HvFP1 results in the delay of normal leaf senescence, but not in the delay of rapid, drought-induced leaf senescence. In addition, the overexpression of HvFP1 suppresses the induction of the ABA-related genes during drought and senescence, e.g., HvNCED, HvS40, HvDhn1. Even though HvFP1 is induced during drought, senescence and the ABA treatment, its overexpression suppresses the ABA regulated genes. This indicates that HvFP1 is acting in a negative feedback loop connected to the ABA signaling. The genome-wide transcriptomic analysis via RNA sequencing revealed that the gain-of-function of HvFP1 positively alters the expression of the genes related to leaf development, photomorphogenesis, photosynthesis and chlorophyll biosynthesis. Interestingly, many of those genes encode proteins with zinc binding domains, implying that HvFP1 may act as zinc supplier via its HMA domain. The results show that HvFP1 is involved in a crosstalk between stress responses and growth control pathways.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11212851