Chasmophyte associated stress tolerant bacteria confer drought resilience to chickpea through efficient nutrient mining and modulation of stress response

• Published in: Scientific reports 2024-05, Vol.14 (1), p.12189-12189, Article 12189
• Main Authors: Das, Sudipta, Chakdar, Hillol, Kumar, Adarsh, Singh, Rajni, Saxena, Anil Kumar
• Format: Article
• Language: English
• Subjects: 631/326/171; 631/326/171/1818; 631/326/2522; Acetic acid; Antioxidants - metabolism; Bacteria; Bacteria - metabolism; Carbon-Carbon Lyases - metabolism; Chasmophyte; Chickpea; Cicer - growth & development; Cicer - microbiology; Cicer - physiology; Drought; Droughts; Enterobacter - metabolism; Enterobacter - physiology; Field tests; Humanities and Social Sciences; Indoleacetic acid; Indoleacetic Acids - metabolism; Inoculation; Legumes; multidisciplinary; Nutrient content; Nutrients - metabolism; Osmotic stress; Plant bacterial diseases; Plant growth promotion; Pseudomonas; Pseudomonas - physiology; Science; Science (multidisciplinary); Stimulants; Stress, Physiological; Temperature tolerance; Water stress
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-58695-3

In the present study, ten (10) selected bacteria isolated from chasmophytic wild Chenopodium were evaluated for alleviation of drought stress in chickpea. All the bacterial cultures were potential P, K and Zn solubilizer. About 50% of the bacteria could produce Indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylate (ACC) deaminase. The bacteria showed wide range of tolerance towards pH, salinity, temperature and osmotic stress. Bacillus paralicheniformis L38, Pseudomonas sp. LN75, Enterobacter hormachei subsp. xiangfengensis LJ89, B. paramycoides L17 and Micrococcus luteus LA9 significantly improved growth and nutrient (N, P, K, Fe and Zn) content in chickpea under water stress during a green house experiment conducted following a completely randomized design (CRD). Application of Microbacterium imperiale LJ10, B. stercoris LN74, Pseudomonas sp. LN75, B. paralicheniformis L38 and E. hormachei subsp. xiangfengensis LJ89 reduced the antioxidant enzymes under water stress. During field experiments conducted following randomized block design (RBD), all the bacterial inoculations improved chickpea yield under water stress. Highest yield (1363 kg ha −1 ) was obtained in plants inoculated with Pseudomonas sp. LN75. Pseudomonas sp. LN75, B. paralicheniformis L38 and E. hormachei subsp. xiangfengensis LJ89 have potential as microbial stimulants to alleviate the water stress in chickpea. To the best of our knowledge this is the first report of using chasmophyte associated bacteria for alleviation of water stress in a crop plant.