Expression and regulation of prostaglandin transporters, ATP-binding cassette, subfamily C, member 1 and 9, and solute carrier organic anion transporter family, member 2A1 and 5A1 in the uterine endometrium during the estrous cycle and pregnancy in pigs

• Published in: Animal bioscience 2017, 30(5), , pp.643-652
• Main Authors: Jang, Hwanhee, Choi, Yohan, Yoo, Inkyu, Han, Jisoo, Kim, Minjeong, Ka, Hakhyun
• Format: Article
• Language: English
• Subjects: Endometrium; Gene expression; Genetic aspects; Observations; Physiological aspects; Pig; Pregnancy; Prostaglandin; Prostaglandin Transporters; Prostaglandins; Swine; Uterus; 축산학
• ISSN: 1011-2367; 2765-0189; 1976-5517; 2765-0235
• DOI: 10.5713/ajas.16.0637

Prostaglandins (PGs) function in various reproductive processes, including luteolysis, maternal pregnancy recognition, conceptus development, and parturition. Our earlier study has shown that PG transporters ATP-binding cassette, subfamily C, member 4 ( ) and solute carrier organic anion transporter family, member 2A1 ( ) are expressed in the uterine endometrium in pigs. Since several other PG transporters such as , , , and are known to be present in the uterine endometrium, this study investigated the expression of these PG transporters in the porcine uterine endometrium and placenta. Uterine endometrial tissues were obtained from gilts on day (D) 12 and D15 of the estrous cycle and days 12, 15, 30, 60, 90, and 114 of pregnancy. , , , and mRNAs were expressed in the uterine endometrium, and levels of expression changed during the estrous cycle and pregnancy. Expression of and mRNAs was localized mainly to luminal and glandular epithelial cells in the uterine endometrium, and chorionic epithelial cells during pregnancy. Conceptuses during early pregnancy and chorioallantoic tissues from mid to late pregnancy also expressed these PG transporters. Estradiol-17β increased the expression of and , but not and mRNAs and increasing doses of interleukin-1β induced the expression of , , and mRNAs in endometrial explant tissues. These data showed that several PG transporters such as , , , and were expressed at the maternal-conceptus interface, suggesting that these PG transporters may play an important role in the establishment and maintenance of pregnancy by regulating PG transport in the uterine endometrium and placenta in pigs.