Protocol for ovariectomy and estradiol replacement in mice

Ovariectomy, involving the surgical removal of ovaries, and estradiol replacement facilitate the understanding of sexual dimorphism-related physiological changes, encompassing reproductive biology, metabolism, and hormone-related diseases. In this study, we present a protocol for conducting ovariect...

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Bibliographic Details
Published in:STAR protocols 2024-03, Vol.5 (1), p.102910-102910, Article 102910
Main Authors: Luengo-Mateos, María, González-Vila, Antía, Torres Caldas, Ana María, Alasaoufi, Ali M., González-Domínguez, Marco, López, Miguel, González-García, Ismael, Barca-Mayo, Olga
Format: Article
Language:English
Subjects:
Animals
Estradiol - pharmacology
Female
Humans
Metabolism
Mice
Model Organisms
Ovariectomy - adverse effects
Ovary - surgery
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Summary:Ovariectomy, involving the surgical removal of ovaries, and estradiol replacement facilitate the understanding of sexual dimorphism-related physiological changes, encompassing reproductive biology, metabolism, and hormone-related diseases. In this study, we present a protocol for conducting ovariectomy and estradiol replacement in mice. We describe steps for performing sham and ovariectomy operations, outline preoperative preparations, and provide details on postoperative care, including analgesia administration and the removal of surgical clips. Additionally, we elaborate on the procedures for performing vehicle and estradiol injections. For complete details on the use and execution of this protocol, please refer to Luengo-Mateos et al.1 [Display omitted] •A guide with detailed procedural steps for performing ovariectomy in mice•Instructions covering pre-ovariectomy preparations and postoperative care•Steps and considerations for administering estradiol to ovariectomized mice Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Ovariectomy, involving the surgical removal of ovaries, and estradiol replacement facilitate the understanding of sexual dimorphism-related physiological changes, encompassing reproductive biology, metabolism, and hormone-related diseases. In this study, we present a protocol for conducting ovariectomy and estradiol replacement in mice. We describe steps for performing sham and ovariectomy operations, outline preoperative preparations, and provide details on postoperative care, including analgesia administration and the removal of surgical clips. Additionally, we elaborate on the procedures for performing vehicle and estradiol injections.
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2024.102910