miR-19b-3p promotes colon cancer proliferation and oxaliplatin-based chemoresistance by targeting SMAD4: validation by bioinformatics and experimental analyses

As a disease with extremely complex molecular mechanisms, many deregulated miRNAs have been identified in colon cancer. Few studies have been performed by using Ingenuity Pathways Analysis (IPA) to predict miRNAs specifically expressed in colon cancer. A characteristic microRNA-target network of col...

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Bibliographic Details
Published in:Journal of experimental & clinical cancer research 2017-09, Vol.36 (1), p.131-131, Article 131
Main Authors: Jiang, Tao, Ye, Ling, Han, Zhongbo, Liu, Yuan, Yang, Yinxue, Peng, Zhihai, Fan, Junwei
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Bioinformatics
Biomarkers
Bone cancer
Caco-2 Cells
Cancer therapies
Cell growth
Cell Line, Tumor
Cell Proliferation
Chemoresistance
Colon cancer
Colorectal cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Computational Biology - methods
Disease Progression
Drug resistance
Drug Resistance, Neoplasm
Drug therapy
Female
Gene Expression Regulation, Neoplastic
Genetic aspects
HCT116 Cells
Health aspects
HT29 Cells
Humans
Male
Medical prognosis
Medical research
Metastasis
Mice
MicroRNA
MicroRNAs
MicroRNAs - genetics
miR-19b-3p
Neoplasm Staging
Neoplasm Transplantation
Organoplatinum Compounds - pharmacology
Oxaliplatin
Physiological aspects
Prognosis
Proliferation
SMAD4
Smad4 Protein - genetics
Surgery
Up-Regulation
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Summary:As a disease with extremely complex molecular mechanisms, many deregulated miRNAs have been identified in colon cancer. Few studies have been performed by using Ingenuity Pathways Analysis (IPA) to predict miRNAs specifically expressed in colon cancer. A characteristic microRNA-target network of colon cancer was explored using IPA. Then the clinical significance of miR-19b-3p was evaluated in 211 colon cancer patients. The roles of miR-19b-3p and its candidate target gene, SMAD4, in colon cancer progression were examined both in vitro and in vivo. Bioinformatics analysis showed that 15 microRNAs screened by IPA were significantly correlated with malignant biological behaviors of colon cancer. miR-19b-3p was the most significantly upregulated candidate based on the validation experiment using 211 colon cancer samples. High expression of miR-19b-3p was significantly associated with high N stage (P 
ISSN:1756-9966
0392-9078
1756-9966
DOI:10.1186/s13046-017-0602-5