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Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study

Sheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of Lactobacillus used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been performed to analyze th...

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Published in:Animals (Basel) 2020-04, Vol.10 (4), p.719
Main Authors: Quereda, Juan J, García-Roselló, Empar, Barba, Marta, Mocé, María L, Gomis, Jesús, Jiménez-Trigos, Estrella, Bataller, Esther, Martínez-Boví, Rebeca, García-Muñoz, Ángel, Gómez-Martín, Ángel
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cited_by cdi_FETCH-LOGICAL-c514t-1ce515e95cf4407ecafc4638c8abd43e747da577c2492e3302d0f8c591793e583
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creator Quereda, Juan J
García-Roselló, Empar
Barba, Marta
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Gomis, Jesús
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Martínez-Boví, Rebeca
García-Muñoz, Ángel
Gómez-Martín, Ángel
description Sheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of Lactobacillus used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been performed to analyze the potential use of probiotics to prevent urogenital infections in sheep. A randomized controlled clinical trial was conducted with 21 one-year-old ewes to develop a model of probiotic infusion in vaginal sponges in order to study their influence in ewe's vaginal microbiota, general health status, fertility and prolificity. Synchronization of estrus was based on intravaginal sponges for 14 days. Bacterial communities (Enterobacteriaceae and lactic acid bacteria) were highly fluctuating over time and between animals. The safety of probiotic infusion (mix of Lactobacillus spp. 60% L. crispatus, 20% L. brevis and 20% L. gasseri) in the vagina of healthy ewes was firstly confirmed. Neutrophils were observed in 80% (8/10) of the control ewes compared to 36% (4/11) of the ewes in the probiotic group 2 days after sponge removal (p = 0.056). Fertility in the control and probiotic groups was 60% (6/10) and 91% (10/11), respectively p = 0.097. These results suggest that Lactobacillus spp. infusion in the ewe's vagina does not affect general health status or fertility.
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Neutrophils were observed in 80% (8/10) of the control ewes compared to 36% (4/11) of the ewes in the probiotic group 2 days after sponge removal (p = 0.056). Fertility in the control and probiotic groups was 60% (6/10) and 91% (10/11), respectively p = 0.097. 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subjects alternative to antibiotics
Communication
dysbiosis
estrus synchronization
Health aspects
lactic acid bacteria
Lactobacillus
Microbiota (Symbiotic organisms)
Probiotics
Sheep
vaginitis
title Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study
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