In Vitro Research Reproducibility: Keeping Up High Standards

• Published in: Frontiers in pharmacology 2019-12, Vol.10, p.1484
• Main Authors: Hirsch, Cordula, Schildknecht, Stefan
• Format: Article
• Language: English
• Subjects: cell culture models; good cell culture practice; new approach methods (NAM); Pharmacology; reproducibility; toxicology
• ISSN: 1663-9812; 1663-9812
• DOI: 10.3389/fphar.2019.01484

Concern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical research. The use of cell-based systems has increasingly replaced research and the application of models enjoys an ever-growing popularity. To avoid repeating past mistakes, high standards of reproducibility and reliability must be established and maintained in the field of biomedical research. Detailed guidance documenting the appropriate handling of cells has been authored, but was received with quite disparate perception by different branches in biomedical research. In that regard, we intend to raise awareness of the reproducibility issue among scientists in all branches of contemporary life science research and their individual responsibility in this matter. We have herein compiled a selection of the most susceptible steps of everyday cell culture routines that have the potential to influence cell quality and recommend practices to minimize the likelihood of poor cell quality impairing reproducibility with modest investment of time and resources.