Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells

• Published in: BMC cancer 2023-09, Vol.23 (1), p.1-12, Article 853
• Main Authors: Wang, Fang, Song, Shuyan, Guo, Bingxuan, Li, Yangyang, Wang, Huijuan, Fu, Shaowei, Wang, Luyue, Zhe, Xiangyi, Li, Hongtao, Li, Dongmei, Shao, Renfu, Pan, Zemin
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Antibodies; Apoptosis; Biomarkers; Cancer; Cancer cells; Caspase-3; Cell cycle; Cell migration; Cell proliferation; Cell survival; Cells; Cervical cancer; Cyclin B1; Development and progression; Diagnosis; DNA methylation; DNA microarrays; E-cadherin; Flow cytometry; Gene expression; Genes; Genetic aspects; Gynecological cancer; Health aspects; Human papillomavirus; Hybridization; Infections; Malignancy; Medical prognosis; Messenger RNA; Metastasis; Migration and proliferation; Molecular modelling; Molecular weight; Polymerase chain reaction; Proteins; Reagents; Risk factors; TCP11 gene; Zonula occludens-1 protein
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-023-11129-1

Cervical cancer is a common gynecological malignancy. Gene microarray found that TCP11 gene was highly expressed in cervical cancer. However, the effect of TCP11 gene on the proliferation, apoptosis and migration of cervical cancer cells and its underlying molecular mechanisms are unclear. GEPIA database, tissue microarray, western blot and qRT-PCR were used to analyze the expression of TCP11 gene in cervical cancer tissues and cells and its relationship with patients' survival rate. The cell cycle and apoptosis were detected by flow cytometry, and the expressions of cell cycle and apoptosis related molecules and EMT-related molecules were detected by Western blot and qRT-PCR. The results showed that TCP11 gene was highly expressed in cervical cancer tissues and cells compared with normal cervical tissues and cells, and its expression was positively correlated with patients' survival rate. The results of proliferation and migration assays showed that TCP11 overexpression inhibited the proliferation and migration of HeLa and SiHa cells. The results showed that TCP11 overexpression blocked the cell cycle of HeLa and SiHa cells, decreased the expression of CDK1 and Cyclin B1, and increased the apoptosis and the expression of caspase-3, cleaved-caspase-3 and cleaved-PARP. TCP11 overexpression increased the protein and mRNA expression of EMT-related molecules ZO-1 and E-cadherin. Conversely, TCP11 knockdown promoted the proliferation of HeLa and SiHa cells and the migration of HeLa cells. TCP11 overexpression significantly inhibited the occurrence and development of cervical cancer cells, it may be a potentially beneficial biomarker for cervical cancer.