Proteomics in the World of Induced Pluripotent Stem Cells

Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating d...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2019-07, Vol.8 (7), p.703
Main Authors: Lindoso, Rafael Soares, Kasai-Brunswick, Tais H, Monnerat Cahli, Gustavo, Collino, Federica, Bastos Carvalho, Adriana, Campos de Carvalho, Antonio Carlos, Vieyra, Adalberto
Format: Article
Language:English
Subjects:
Algorithms
Amino acids
Bioinformatics
Cell culture
Cell Differentiation
Chromatography
Data analysis
differentiation
Embryo cells
embryonic stem cells
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Enzymes
Humans
Identification
induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Localization
Mass spectrometry
Organoids
Peptides
Pluripotency
Proteins
Proteome - metabolism
Proteomes
Proteomics
Proteomics - methods
Review
Scientific imaging
Stem cells
Transcriptome
Transcriptomes
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Summary:Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and the differentiation process to the diverse cell types that form organisms. This review covers the evolution of conceptual and methodological strategies in proteomics; briefly describes the generation of iPSC from a historical perspective, the state-of-the-art of iPSC-based proteomics; and compares data on the proteome and transcriptome of iPSC to that of embryonic stem cells (ESC). Finally, proteomics of healthy and diseased cells and organoids differentiated from iPSC are analyzed.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells8070703