A methodology for the production of microfabricated electrospun membranes for the creation of new skin regeneration models

The continual renewal of the epidermis is thought to be related to the presence of populations of epidermal stem cells residing in physically protected microenvironments (rete ridges) directly influenced by the presence of mesenchymal fibroblasts. Current skin in vitro models do acknowledge the infl...

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Bibliographic Details
Published in:Journal of tissue engineering 2018-01, Vol.9, p.2041731418799851-2041731418799851
Main Authors: Asencio, Ilida Ortega, Mittar, Shweta, Sherborne, Colin, Raza, Ahtasham, Claeyssens, Frederik, MacNeil, Sheila
Format: Article
Language:English
Subjects:
Acellular Approaches for Regenerative Medicine: Driving Biology without the Cell
Biomaterials
Biomedical materials
Cell culture
Electrospinning
Epidermis
Epithelial cells
Exploration
Fibroblasts
Keratinocytes
Membranes
Mesenchyme
Microenvironments
Polyethylene glycol
Regeneration
Scaffolds
Skin
Stem cells
Three dimensional models
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Summary:The continual renewal of the epidermis is thought to be related to the presence of populations of epidermal stem cells residing in physically protected microenvironments (rete ridges) directly influenced by the presence of mesenchymal fibroblasts. Current skin in vitro models do acknowledge the influence of stromal fibroblasts in skin reorganisation but the study of the effect of the rete ridge-microenvironment on epidermal renewal still remains a rich topic for exploration. We suggest there is a need for the development of new in vitro models in which to study epithelial stem cell behaviour prior to translating these models into the design of new cell-free biomaterial devices for skin reconstruction. In this study, we aimed to develop new prototype epidermal-like layers containing pseudo-rete ridge structures for studying the effect of topographical cues on epithelial cell behaviour. The models were designed using a range of three-dimensional electrospun microfabricated scaffolds. This was achieved via the utilisation of polyethylene glycol diacrylate to produce a reusable template over which poly(3-hydrroxybutyrate-co-3-hydroxyvalerate) was electrospun. Initial investigations studied the behaviour of keratinocytes cultured on models using plain scaffolds (without the presence of intricate topography) versus keratinocytes cultured on scaffolds containing microfeatures.
ISSN:2041-7314
2041-7314
DOI:10.1177/2041731418799851