Fluorescent Tagged Vaccinia Virus Genome Allows Rapid and Efficient Measurement of Oncolytic Potential and Discovery of Oncolytic Modulators

As a live biologic agent, oncolytic vaccinia virus has the ability to target and selectively amplify at tumor sites. We have previously reported that deletion of thymidine kinase and ribonucleotide reductase genes in vaccinia virus can increase the safety and efficacy of the virus. Here, to allow di...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines 2020-11, Vol.8 (12), p.543
Main Authors: Gallardo, Franck, Schmitt, Doris, Brandely, Renée, Brua, Catherine, Silvestre, Nathalie, Findeli, Annie, Foloppe, Johann, Top, Sokunthea, Kappler-Gratias, Sandrine, Quentin-Froignant, Charlotte, Morin, Renaud, Lagarde, Jean-Michel, Bystricky, Kerstin, Bertagnoli, Stéphane, Erbs, Philippe
Format: Article
Language:English
Subjects:
Adenoviruses
Cancer
Deoxyribonucleic acid
DNA
DNA biosynthesis
fluorescence labeling
Genomes
Immunotherapy
Infections
Kinases
Life Sciences
live cell imaging
Monoclonal antibodies
Nucleotide sequence
Oncolysis
oncolytic vaccinia virus
Plasmids
poxvirus modulators
Thymidine
Thymidine kinase
Tumor cells
Viruses
Visualization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:As a live biologic agent, oncolytic vaccinia virus has the ability to target and selectively amplify at tumor sites. We have previously reported that deletion of thymidine kinase and ribonucleotide reductase genes in vaccinia virus can increase the safety and efficacy of the virus. Here, to allow direct visualization of the viral genome in living cells, we incorporated the ANCH target sequence and the OR3-Santaka gene in the double-deleted vaccinia virus. Infection of human tumor cells with ANCHOR3-tagged vaccinia virus enables visualization and quantification of viral genome dynamics in living cells. The results show that the ANCHOR technology permits the measurement of the oncolytic potential of the double deleted vaccinia virus. Quantitative analysis of infection kinetics and of viral DNA replication allow rapid and efficient identification of inhibitors and activators of oncolytic activity. Our results highlight the potential application of the ANCHOR technology to track vaccinia virus and virtually any kind of poxvirus in living cells.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines8120543