Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys

Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-rea...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2018-09, Vol.51 (5), p.665-669
Main Authors: Santos, Anderson Pacheco Dos, Carvalho, Maria Esther de, Meirelles, Luciana Regina, Andrade Junior, Heitor Franco de
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Protozoan - blood
Antibody Affinity - immunology
Avidity
Biomarkers - chemistry
Brazil - epidemiology
Chagas disease
Chagas Disease - complications
Chagas Disease - diagnosis
Chagas Disease - epidemiology
Chagas Disease - immunology
Cross Reactions - immunology
Cross-reactivity
Dilution
ELISA
Enzyme-Linked Immunosorbent Assay
Epidemiology
Humans
IFAT
IgG antibody avidity
Immunoassays
Immunoglobulin G
Immunoglobulin G - blood
Leishmaniasis
Leishmaniasis - complications
Leishmaniasis - diagnosis
Leishmaniasis - epidemiology
Leishmaniasis - immunology
Parasitic diseases
Population Surveillance
Sensitivity and Specificity
Seroepidemiology
Serology
Tropical diseases
TROPICAL MEDICINE
Urea
Urea - chemistry
Urea - pharmacology
Ureas
Vector-borne diseases
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description Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
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subjects Antibodies
Antibodies, Protozoan - blood
Antibody Affinity - immunology
Avidity
Biomarkers - chemistry
Brazil - epidemiology
Chagas disease
Chagas Disease - complications
Chagas Disease - diagnosis
Chagas Disease - epidemiology
Chagas Disease - immunology
Cross Reactions - immunology
Cross-reactivity
Dilution
ELISA
Enzyme-Linked Immunosorbent Assay
Epidemiology
Humans
IFAT
IgG antibody avidity
Immunoassays
Immunoglobulin G
Immunoglobulin G - blood
Leishmaniasis
Leishmaniasis - complications
Leishmaniasis - diagnosis
Leishmaniasis - epidemiology
Leishmaniasis - immunology
Parasitic diseases
Population Surveillance
Sensitivity and Specificity
Seroepidemiology
Serology
Tropical diseases
TROPICAL MEDICINE
Urea
Urea - chemistry
Urea - pharmacology
Ureas
Vector-borne diseases
title Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
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