Chemical Fingerprinting of Cryptic Species and Genetic Lineages of Aneura pinguis (L.) Dumort. (Marchantiophyta, Metzgeriidae)

(L.) Dumort. is a representative of the simple thalloid liverworts, one of the three main types of liverwort gametophytes. According to classical taxonomy, represents one morphologically variable species; however, genetic data reveal that this species is a complex consisting of 10 cryptic species (n...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-02, Vol.26 (4), p.1180
Main Authors: Wawrzyniak, Rafał, Wasiak, Wiesław, Jasiewicz, Beata, Bączkiewicz, Alina, Buczkowska, Katarzyna
Format: Article
Language:English
Subjects:
Aneura pinguis
Aquatic plants
Chemical compounds
Chemical fingerprinting
chemical markers
Cluster analysis
Cryptic species
Deoxyribonucleic acid
DNA
DNA fingerprinting
Gametophytes
Gas Chromatography-Mass Spectrometry
genetic lineages
Genetic Speciation
HS-SPME/GC-MS
liverworts
Marchantia - genetics
Markers
Phylogeny
Principal components analysis
Statistical analysis
Statistical methods
Taxonomy
Volatile compounds
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Summary:(L.) Dumort. is a representative of the simple thalloid liverworts, one of the three main types of liverwort gametophytes. According to classical taxonomy, represents one morphologically variable species; however, genetic data reveal that this species is a complex consisting of 10 cryptic species (named by letters from A to J), of which four are further subdivided into two or three evolutionary lineages. The objective of this work was to develop an efficient method for the characterisation of plant material using marker compounds. The volatile chemical constituents of cryptic species within the liverwort were analysed by GC-MS. The compounds were isolated from plant material using the HS-SPME technique. Of the 66 compounds examined, 40 were identified. Of these 40 compounds, nine were selected for use as marker compounds of individual cryptic species of . A guide was then developed that clarified how these markers could be used for the rapid identification of the genetic lineages of . Multivariate statistical analyses (principal component and cluster analysis) revealed that the chemical compounds in made it possible to distinguish individual cryptic species (including genetic lineages), with the exception of cryptic species G and H. The classification of samples based on the volatile compounds by cluster analysis reflected phylogenetic relationships between cryptic species and genetic lineages of revealed based on molecular data.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26041180