Lutein Encapsulated in PLGA-Phospholipid Nano-Carrier Effectively Mitigates Cytokines by Inhibiting Tumor Necrosis Factor TNF-α and Nuclear Factor NF- κ B in Mice Retina

• Published in: Journal of functional biomaterials 2023-04, Vol.14 (4), p.197
• Main Authors: Arunkumar, Ranganathan, Baskaran, Vallikannan
• Format: Article
• Language: English
• Subjects: anti-inflammation; Anti-inflammatory agents; Bioavailability; Biological activity; Carbonyl compounds; Carbonyls; carotenoids; Complications; Glycolic acid; Inflammation; Lipopolysaccharides; Lutein; nanocarrier; NF-κB protein; Nitric oxide; Nitrites; Oxidation; Phospholipids; PLGA; Polyethylene glycol; Polylactide-co-glycolide; Retina; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb14040197

Lutein, a photo- and thermo-labile macular pigment, prevents the retina from suffering ocular inflammation with its antioxidant and anti-inflammatory activity. However, its biological activity is poor due to poor solubility and bioavailability. Therefore, we developed a PLGA NCs (+PL), (poly (lactic- -glycolic acid) nanocarrier with phospholipid) to improve the biological availability and bioefficacy of lutein in the retina of lipopolysaccharide (LPS)-induced lutein-devoid (LD) mice. The effect of lutein-loaded NCs with/without PL was studied in comparison with micellar lutein. The induction of inflammation by LPS significantly increased the production of nitrites in the LPS-induced group, revealing higher levels of nitric oxide (NO) in the serum (760%) and retina (891%) compared to the control group. Malondialdehyde (MDA) levels in the serum (93%) and retina (205%) of the LPS-induced group were higher compared to the control group. LPS induction resulted in increased protein carbonyls in the serum (481%) and retina (487%) of the LPS group compared to the control group. Further, to conclude, lutein-PLGA NCs (+PL) effectively down-regulated inflammatory complications in the retina.