Efficient Bioremediation of Petroleum-Contaminated Soil by Immobilized Bacterial Agent of Gordonia alkanivorans W33

• Published in: Bioengineering (Basel) 2023-05, Vol.10 (5), p.561
• Main Authors: Yang, Yong, Zhang, Wanze, Zhang, Zhanwei, Yang, Ting, Xu, Zhuo, Zhang, Chuanbo, Guo, Bing, Lu, Wenyu
• Format: Article
• Language: English
• Subjects: Activated carbon; Bacteria; Batch culture; Biodegradation; Bioengineering; Bioremediation; Calcium chloride; Cell culture; Degradation; Fermentation; Gordonia; Gordonia alkanivorans W33; Hydrocarbons; Immobilization; Magnesium chloride; microbial immobilization; Microorganisms; Petroleum; petroleum-contaminated soil; Pollution; Response surface methodology; Soil contamination; Soil microorganisms; Soil pollution; Soils; Surface analysis (chemical); Vermiculite
• ISSN: 2306-5354; 2306-5354
• DOI: 10.3390/bioengineering10050561

In this article, we report a method for preparing an immobilized bacterial agent of petroleum-degrading bacteria W33 by combining high-density fermentation and bacterial immobilization technology and testing its bioremediation effect on petroleum-contaminated soil. After determining the optimal combination of MgCl , CaCl concentration, and culture time in the fermentation conditions by conducting a response surface analysis, the cell concentration reached 7.48 × 10 CFU/mL by 5 L fed-batch fermentation. The W33-vermiculite-powder-immobilized bacterial agent mixed with sophorolipids and rhamnolipids in a weight ratio of 9:10 was used for the bioremediation of petroleum-contaminated soil. After 45 days of microbial degradation, 56.3% of the petroleum in the soil with 20,000 mg/kg petroleum content was degraded, and the average degradation rate reached 250.2 mg/kg/d.