Postprandial sleep mechanics in Drosophila

Food consumption is thought to induce sleepiness. However, little is known about how postprandial sleep is regulated. Here, we simultaneously measured sleep and food intake of individual flies and found a transient rise in sleep following meals. Depending on the amount consumed, the effect ranged fr...

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Bibliographic Details
Published in:eLife 2016-11, Vol.5
Main Authors: Murphy, Keith R, Deshpande, Sonali A, Yurgel, Maria E, Quinn, James P, Weissbach, Jennifer L, Keene, Alex C, Dawson-Scully, Ken, Huber, Robert, Tomchik, Seth M, Ja, William W
Format: Article
Language:English
Subjects:
Animals
behavior
Diet
Drosophila
Drosophila - physiology
Eating
Experiments
feeding
Food
Food consumption
Food intake
Insects
Meals
Metabolism
neurogenetics
Neurons
Neurons - physiology
Neuroscience
Neurosciences
nutrition
Physiological aspects
Postprandial Period
Proteins
RNA-mediated interference
Sleep
Sleep and wakefulness
Sucrose
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Summary:Food consumption is thought to induce sleepiness. However, little is known about how postprandial sleep is regulated. Here, we simultaneously measured sleep and food intake of individual flies and found a transient rise in sleep following meals. Depending on the amount consumed, the effect ranged from slightly arousing to strongly sleep inducing. Postprandial sleep was positively correlated with ingested volume, protein, and salt-but not sucrose-revealing meal property-specific regulation. Silencing of leucokinin receptor (Lkr) neurons specifically reduced sleep induced by protein consumption. Thermogenetic stimulation of leucokinin (Lk) neurons decreased whereas Lk downregulation by RNAi increased postprandial sleep, suggestive of an inhibitory connection in the Lk-Lkr circuit. We further identified a subset of non-leucokininergic cells proximal to Lkr neurons that rhythmically increased postprandial sleep when silenced, suggesting that these cells are cyclically gated inhibitory inputs to Lkr neurons. Together, these findings reveal the dynamic nature of postprandial sleep.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.19334