Fine Mapping and Candidate Gene Analysis of Resistance Gene RSC3Q to Soybean mosaic virus in Qihuang 1

• Published in: Journal of Integrative Agriculture 2014-12, Vol.13 (12), p.2608-2615
• Main Author: Zheng gui-jie Yang Yong-qing Ma Ying Yang Xiao-feng Chen Shan-yu Ren Rui Wang Da-gang Yang Zhong-lu Zhi hai-jian
• Format: Article
• Language: English
• Subjects: F2群体; fine mapping; near isogenic lines (NILs); qRT-PCR; residual heterozygous line (RHLs); resistance gene; Soybean mosaic virus (SMV); Soybean mosaic virus (SMV) resistance gene fine mapping residual heterozygous line (RHLs) near isogenic lines (nILs) qRT-PCR; 候选基因; 分子标记辅助选择; 基因分析; 大豆花叶病毒; 抗性基因; 简单重复序列; 精细定位
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(13)60738-8

Soybean mosaic virus （SMV） disease is one of the most destructive viral diseases in soybean （Glycine max （L.） Merr.）. SMV strain SC3 is the major prevalent strain in huang-huai and Yangtze valleys, China. The soybean cultivar Qihuang 1 is of a rich resistance spectrum and has a wide range of application in breeding programs in China. In this study, F1, F2 and F2：3 from Qihuang 1×nannong 1138-2 were used to study inheritance and linkage mapping of the SC3 resistance gene in Qihuang 1. The secondary F2 population and near isogenic lines （nILs） derived from residual heterozygous lines （RhLs） of Qihuang 1×nannong 1138-2 were separatively used in the ifne mapping and candidate gene analysis of the resistance gene. Results indicated that a single dominant gene （designated RSC3Q） controls resistance, which was located on chromosome 13. Two genomic-simple sequence repeat （SSR） markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136 were found lfanking the two sides of the RSC3Q. The interval between the two markers was 651 kb. Quantitative real-time PCR analysis of the candidate genes showed that ifve genes （Glyma13g25730, 25750, 25950, 25970 and 26000） were likely involved in soybean SMV resistance. These results would have utility in cloning of RSC3Q resistance candidate gene and marker-assisted selection （MaS） in resistance breeding to SMV.