Immunohistochemical and ultrastructural study of the lamellae of oocytes in atretic follicles in relation to different processes of cell death

Atresia is the process through which non-selectable oocytes are eliminated; it involves apoptosis and/or autophagy. This study used immunohistochemical and ultrastructural techniques to characterize the lamellae present in the cytoplasm of oocytes in follicles in the process of atresia in prepuberta...

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Bibliographic Details
Published in:European journal of histochemistry 2015-08, Vol.59 (3), p.2535-2535
Main Authors: Escobar, M L, Echeverría, O M, García, G, Ortíz, R, Vázquez-Nin, G H
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Atresia
Autophagy
cytoskeleton
Female
Follicular Atresia - metabolism
Immunohistochemistry
Microscopy, Electron, Transmission
Oocytes - metabolism
Oocytes - ultrastructure
Original Paper
Ovarian Follicle - metabolism
Ovarian Follicle - ultrastructure
Rats
tubulin
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Summary:Atresia is the process through which non-selectable oocytes are eliminated; it involves apoptosis and/or autophagy. This study used immunohistochemical and ultrastructural techniques to characterize the lamellae present in the cytoplasm of oocytes in follicles in the process of atresia in prepubertal and adult Wistar rats. The results indicate that the lamellae are positive to tubulin and myosin immunodetection under light and electron microscopy. Labeling is greater with anti-tubulin and lesser with anti-myosin. Our observations indicate that lamellae are present in oocytes at the initial antral stage in prepubertal rats; that is, from day 14 post-birth to adult age. We were able to determine that the increase in altered lamellae principally occurs in the apoptotic cells rather than in the autophagic cells.
ISSN:1121-760X
2038-8306
DOI:10.4081/ejh.2015.2535