Chinese hamster ovary cells overexpressing the oxysterol binding protein (OSBP) display enhanced synthesis of sphingomyelin in response to 25-hydroxycholesterol

25-Hydroxycholesterol negatively regulates cholesterol synthesis and activates cholesterol esterification in a variety of cultured cells. Concurrent with these effects, 25-hydroxycholesterol also stimulates the synthesis of sphingomyelin in Chinese hamster ovary (CHO)-K1 cells. The role of oxysterol...

Full description

Saved in:
Bibliographic Details
Published in:Journal of lipid research 1999-01, Vol.40 (1), p.109-116
Main Authors: Lagace, T A, Byers, D M, Cook, H W, Ridgway, N D
Format: Article
Language:English
Subjects:
25-hydroxycholesterol
Animals
ceramide
Ceramides - metabolism
CHO Cells
Cholesterol - biosynthesis
Cholesterol Esters - biosynthesis
Cricetinae
Gene Expression
Golgi Apparatus - metabolism
Hydroxycholesterols - pharmacology
Kinetics
oxysterol binding protein
Receptors, Steroid - genetics
Receptors, Steroid - metabolism
sphingomyelin
Sphingomyelins - biosynthesis
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:25-Hydroxycholesterol negatively regulates cholesterol synthesis and activates cholesterol esterification in a variety of cultured cells. Concurrent with these effects, 25-hydroxycholesterol also stimulates the synthesis of sphingomyelin in Chinese hamster ovary (CHO)-K1 cells. The role of oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol, in activation of SM synthesis was assessed by overexpression in CHO-K1 cells. When compared to mock transfected controls, three CHO-K1 clones overexpressing OSBP by 10- to 15-fold displayed a 2- to 3-fold enhancement of [3H]serine incorporation into sphingomyelin when treated with 25-hydroxycholesterol. Closer examination of one of these clones (CHO-OSBP cells) revealed a >8.5-fold stimulation of sphingomyelin synthesis after a 6-h treatment with 25-hydroxycholesterol compared to 3.5-fold in controls, slightly higher basal levels of sphingomyelin synthesis, and a more rapid response to 25-hydroxycholesterol. [3H]serine incorporation into phosphatidylserine, phosphatidylethanolamine, ceramide, or glucosylceramide was affected by
ISSN:0022-2275
DOI:10.1016/s0022-2275(20)33345-9