Orthotopic liver transplantation from cardiac death donors in the mouse: a new model and evaluation of cardiac death time

• Published in: Iranian journal of basic medical sciences 2017-06, Vol.20 (6), p.683-689
• Main Authors: Liu, Zhenzhen, Pan, Ning, Lv, Xiangwei, Li, Song, Wang, Liming, Liu, Qinlong
• Format: Article
• Language: English
• Subjects: Animal model; Liver; Liver transplantation; Liver transplants; Nitric oxide; Original; Primary graft dysfunction; Reperfusion injury; Tumor necrosis factor-TNF; Warm ischemia time
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/IJBMS.2017.8838

The goal of this research was to develop a mouse orthotopic liver transplantation (LTx) model from donor-after-cardiac-death (DCD) grafts. Mice were randomly assigned to the experimental group or the sham group. The mice in the experimental group were divided into three groups according to the warm ischemia time (WIT) of liver graft: normal LTx, WIT 30 minute (min) +LTx and WIT 45 min +LTx. The descending aorta was clamped using a miniature aortic clamp to simulate cardiac arrest in the DCD grafts. Subsequently, the grafts were orthotopically transplanted into C57BL/6 mice. The 7-day survival rate, serum alanine aminotransferase (ALT), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6) mRNA level, tumor necrosis factor-alpha (TNF-α) mRNA level, as well as hepatic pathologic alterations were observed. The 7-day survival rate was markedly lower in the WIT 45 min+LTx group than that in the normal LTx group (25% versus 100%, -value0.05). Serum ALT level of WIT 45 min+LTx group was markedly higher than that of normal LTx and WIT 30 min+LTx group ( -value