Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD

Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compa...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2018-01, Vol.2018 (2018), p.1-7
Main Authors: Schou-Pedersen, Anne Marie Voigt, Lykkesfeldt, J.
Format: Article
Language:English
Subjects:
Acetonitrile
Ammonia
Atherosclerosis
Biochemistry
Biomarkers
Blood plasma
Calibration
Capillary electrophoresis
Chromatography
Comparative analysis
Fluorescence
High performance liquid chromatography
Linearity
Liquid chromatography
Mass spectrometry
Mathematical analysis
Methods
Nitric oxide
Plasma
Potassium
Scientific imaging
Studies
Urine
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Summary:Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compared regarding the quantification of L-arginine and ADMA in human plasma: (A) protein precipitation (PP) based on aqueous trichloroacetic acid (TCA), (B) PP using a mixture of ammonia and acetonitrile, and (C) solid-phase extraction (SPE). The samples were analysed by using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The analytical performance of (A) was comparable with that of (C), demonstrating recoveries of >90%, coefficient of variations (CVs, %) of 0.994), precision (
ISSN:2090-8865
2090-8873
DOI:10.1155/2018/6148515