Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD

Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compa...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical methods in chemistry 2018-01, Vol.2018 (2018), p.1-7
Main Authors: Schou-Pedersen, Anne Marie Voigt, Lykkesfeldt, J.
Format: Article
Language:English
Subjects:
Acetonitrile
Ammonia
Atherosclerosis
Biochemistry
Biomarkers
Blood plasma
Calibration
Capillary electrophoresis
Chromatography
Comparative analysis
Fluorescence
High performance liquid chromatography
Linearity
Liquid chromatography
Mass spectrometry
Mathematical analysis
Methods
Nitric oxide
Plasma
Potassium
Scientific imaging
Studies
Urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3
cites cdi_FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3
container_end_page 7
container_issue 2018
container_start_page 1
container_title Journal of analytical methods in chemistry
container_volume 2018
creator Schou-Pedersen, Anne Marie Voigt
Lykkesfeldt, J.
description Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compared regarding the quantification of L-arginine and ADMA in human plasma: (A) protein precipitation (PP) based on aqueous trichloroacetic acid (TCA), (B) PP using a mixture of ammonia and acetonitrile, and (C) solid-phase extraction (SPE). The samples were analysed by using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The analytical performance of (A) was comparable with that of (C), demonstrating recoveries of >90%, coefficient of variations (CVs, %) of 0.994), precision (
doi_str_mv 10.1155/2018/6148515
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_a7481d57ac52427297062c2bdbdbac55</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A587142191</galeid><doaj_id>oai_doaj_org_article_a7481d57ac52427297062c2bdbdbac55</doaj_id><sourcerecordid>A587142191</sourcerecordid><originalsourceid>FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3</originalsourceid><addsrcrecordid>eNqNkl9v0zAUxSMEYtPYG8_IEi9IWzbbjR3nBanqGJ1UiaJtz9GNc9O6SuxiJ6B-LT4hLimFISERP-Tqnt89_qOTJK8ZvWJMiGtOmbqWLFOCiWfJKacFTZXKJ8-PtRQnyXkIGxo_KSij4mVywotMZZxlp8n3meu24E1wlriGPKw9IrmHbtsiWXqMEvQmakvvNNaDx0Aa50m_RvJ5ANubxuiRiNOLdOpXxhqLl2Qadl2HvTea3JhYrHctHEWwNbn_p06MJfOhg7hrC6ED8hiMXZH5cjFLbxc3r5IXDbQBzw__s-Tx9sPDbJ4uPn28m00XqRa57FOgRSMKLQopkU-UypiCIqtQCcFiLatCQYaiRs2R1nyS6ZpOUFe5rBohimpyltyNvrWDTbn1pgO_Kx2Y8mfD-VUJvje6xRLyTLFa5KAFz3jOi5xKrnlVxxV7Inq9H722Q9VhrdH2Htonpk8Va9blyn0thWJSCRkN3h0MvPsyYOjLzgSNbQsW3RBKTqlSSuRURfTtX-jGDd7Gp9pTtFDxjCxSVyO1gngBYxsX99Vx1dgZ7Sw2JvanQuUsJqXYD1yOA9q7EDw2x9MzWu7DWO7DWB7CGPE3f974CP-KXgQuRmBtbA3fzH_aYWSwgd80y2XG5OQHaD_yDA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2000985241</pqid></control><display><type>article</type><title>Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD</title><source>EBSCOhost Business Source Ultimate</source><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central(OpenAccess)</source><source>Wiley Open Access</source><source>Free Full-Text Journals in Chemistry</source><creator>Schou-Pedersen, Anne Marie Voigt ; Lykkesfeldt, J.</creator><contributor>Sklenarova, Hana ; Hana Sklenarova</contributor><creatorcontrib>Schou-Pedersen, Anne Marie Voigt ; Lykkesfeldt, J. ; Sklenarova, Hana ; Hana Sklenarova</creatorcontrib><description>Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compared regarding the quantification of L-arginine and ADMA in human plasma: (A) protein precipitation (PP) based on aqueous trichloroacetic acid (TCA), (B) PP using a mixture of ammonia and acetonitrile, and (C) solid-phase extraction (SPE). The samples were analysed by using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The analytical performance of (A) was comparable with that of (C), demonstrating recoveries of &gt;90%, coefficient of variations (CVs, %) of &lt;8, and a resolution (Rs) between ADMA and symmetric dimethylarginine (SDMA) of 1.2. (B) was disregarded due to recoveries below 75%. (A) was validated with good results regarding linearity (&gt;0.994), precision (&lt;5%), and sensitivity (lower limit of quantification (LLOQ)) of 0.14 µM and 12 nM for L-arginine and ADMA, respectively. Due to the simplicity and speed of procedure (A), this approach may serve as preferred sample preparation of human plasma samples before HPLC-FLD in providing important information regarding elevated ADMA concentrations.</description><identifier>ISSN: 2090-8865</identifier><identifier>EISSN: 2090-8873</identifier><identifier>DOI: 10.1155/2018/6148515</identifier><identifier>PMID: 29484214</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Acetonitrile ; Ammonia ; Atherosclerosis ; Biochemistry ; Biomarkers ; Blood plasma ; Calibration ; Capillary electrophoresis ; Chromatography ; Comparative analysis ; Fluorescence ; High performance liquid chromatography ; Linearity ; Liquid chromatography ; Mass spectrometry ; Mathematical analysis ; Methods ; Nitric oxide ; Plasma ; Potassium ; Scientific imaging ; Studies ; Urine</subject><ispartof>Journal of analytical methods in chemistry, 2018-01, Vol.2018 (2018), p.1-7</ispartof><rights>Copyright © 2018 Anne Marie Voigt Schou-Pedersen and Jens Lykkesfeldt.</rights><rights>COPYRIGHT 2018 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2018 Anne Marie Voigt Schou-Pedersen and Jens Lykkesfeldt.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Anne Marie Voigt Schou-Pedersen and Jens Lykkesfeldt. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3</citedby><cites>FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3</cites><orcidid>0000-0002-6514-8407</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2000985241/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2000985241?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29484214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sklenarova, Hana</contributor><contributor>Hana Sklenarova</contributor><creatorcontrib>Schou-Pedersen, Anne Marie Voigt</creatorcontrib><creatorcontrib>Lykkesfeldt, J.</creatorcontrib><title>Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD</title><title>Journal of analytical methods in chemistry</title><addtitle>J Anal Methods Chem</addtitle><description>Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compared regarding the quantification of L-arginine and ADMA in human plasma: (A) protein precipitation (PP) based on aqueous trichloroacetic acid (TCA), (B) PP using a mixture of ammonia and acetonitrile, and (C) solid-phase extraction (SPE). The samples were analysed by using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The analytical performance of (A) was comparable with that of (C), demonstrating recoveries of &gt;90%, coefficient of variations (CVs, %) of &lt;8, and a resolution (Rs) between ADMA and symmetric dimethylarginine (SDMA) of 1.2. (B) was disregarded due to recoveries below 75%. (A) was validated with good results regarding linearity (&gt;0.994), precision (&lt;5%), and sensitivity (lower limit of quantification (LLOQ)) of 0.14 µM and 12 nM for L-arginine and ADMA, respectively. Due to the simplicity and speed of procedure (A), this approach may serve as preferred sample preparation of human plasma samples before HPLC-FLD in providing important information regarding elevated ADMA concentrations.</description><subject>Acetonitrile</subject><subject>Ammonia</subject><subject>Atherosclerosis</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Blood plasma</subject><subject>Calibration</subject><subject>Capillary electrophoresis</subject><subject>Chromatography</subject><subject>Comparative analysis</subject><subject>Fluorescence</subject><subject>High performance liquid chromatography</subject><subject>Linearity</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mathematical analysis</subject><subject>Methods</subject><subject>Nitric oxide</subject><subject>Plasma</subject><subject>Potassium</subject><subject>Scientific imaging</subject><subject>Studies</subject><subject>Urine</subject><issn>2090-8865</issn><issn>2090-8873</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl9v0zAUxSMEYtPYG8_IEi9IWzbbjR3nBanqGJ1UiaJtz9GNc9O6SuxiJ6B-LT4hLimFISERP-Tqnt89_qOTJK8ZvWJMiGtOmbqWLFOCiWfJKacFTZXKJ8-PtRQnyXkIGxo_KSij4mVywotMZZxlp8n3meu24E1wlriGPKw9IrmHbtsiWXqMEvQmakvvNNaDx0Aa50m_RvJ5ANubxuiRiNOLdOpXxhqLl2Qadl2HvTea3JhYrHctHEWwNbn_p06MJfOhg7hrC6ED8hiMXZH5cjFLbxc3r5IXDbQBzw__s-Tx9sPDbJ4uPn28m00XqRa57FOgRSMKLQopkU-UypiCIqtQCcFiLatCQYaiRs2R1nyS6ZpOUFe5rBohimpyltyNvrWDTbn1pgO_Kx2Y8mfD-VUJvje6xRLyTLFa5KAFz3jOi5xKrnlVxxV7Inq9H722Q9VhrdH2Htonpk8Va9blyn0thWJSCRkN3h0MvPsyYOjLzgSNbQsW3RBKTqlSSuRURfTtX-jGDd7Gp9pTtFDxjCxSVyO1gngBYxsX99Vx1dgZ7Sw2JvanQuUsJqXYD1yOA9q7EDw2x9MzWu7DWO7DWB7CGPE3f974CP-KXgQuRmBtbA3fzH_aYWSwgd80y2XG5OQHaD_yDA</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Schou-Pedersen, Anne Marie Voigt</creator><creator>Lykkesfeldt, J.</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley &amp; Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6514-8407</orcidid></search><sort><creationdate>20180101</creationdate><title>Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD</title><author>Schou-Pedersen, Anne Marie Voigt ; Lykkesfeldt, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetonitrile</topic><topic>Ammonia</topic><topic>Atherosclerosis</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Blood plasma</topic><topic>Calibration</topic><topic>Capillary electrophoresis</topic><topic>Chromatography</topic><topic>Comparative analysis</topic><topic>Fluorescence</topic><topic>High performance liquid chromatography</topic><topic>Linearity</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mathematical analysis</topic><topic>Methods</topic><topic>Nitric oxide</topic><topic>Plasma</topic><topic>Potassium</topic><topic>Scientific imaging</topic><topic>Studies</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schou-Pedersen, Anne Marie Voigt</creatorcontrib><creatorcontrib>Lykkesfeldt, J.</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East &amp; Africa Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of analytical methods in chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schou-Pedersen, Anne Marie Voigt</au><au>Lykkesfeldt, J.</au><au>Sklenarova, Hana</au><au>Hana Sklenarova</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD</atitle><jtitle>Journal of analytical methods in chemistry</jtitle><addtitle>J Anal Methods Chem</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>2090-8865</issn><eissn>2090-8873</eissn><abstract>Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compared regarding the quantification of L-arginine and ADMA in human plasma: (A) protein precipitation (PP) based on aqueous trichloroacetic acid (TCA), (B) PP using a mixture of ammonia and acetonitrile, and (C) solid-phase extraction (SPE). The samples were analysed by using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The analytical performance of (A) was comparable with that of (C), demonstrating recoveries of &gt;90%, coefficient of variations (CVs, %) of &lt;8, and a resolution (Rs) between ADMA and symmetric dimethylarginine (SDMA) of 1.2. (B) was disregarded due to recoveries below 75%. (A) was validated with good results regarding linearity (&gt;0.994), precision (&lt;5%), and sensitivity (lower limit of quantification (LLOQ)) of 0.14 µM and 12 nM for L-arginine and ADMA, respectively. Due to the simplicity and speed of procedure (A), this approach may serve as preferred sample preparation of human plasma samples before HPLC-FLD in providing important information regarding elevated ADMA concentrations.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29484214</pmid><doi>10.1155/2018/6148515</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-6514-8407</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2090-8865
ispartof Journal of analytical methods in chemistry, 2018-01, Vol.2018 (2018), p.1-7
issn 2090-8865
2090-8873
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_a7481d57ac52427297062c2bdbdbac55
source EBSCOhost Business Source Ultimate; Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central(OpenAccess); Wiley Open Access; Free Full-Text Journals in Chemistry
subjects Acetonitrile
Ammonia
Atherosclerosis
Biochemistry
Biomarkers
Blood plasma
Calibration
Capillary electrophoresis
Chromatography
Comparative analysis
Fluorescence
High performance liquid chromatography
Linearity
Liquid chromatography
Mass spectrometry
Mathematical analysis
Methods
Nitric oxide
Plasma
Potassium
Scientific imaging
Studies
Urine
title Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T16%3A29%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20Three%20Sample%20Preparation%20Procedures%20for%20the%20Quantification%20of%20L-Arginine,%20Asymmetric%20Dimethylarginine,%20and%20Symmetric%20Dimethylarginine%20in%20Human%20Plasma%20Using%20HPLC-FLD&rft.jtitle=Journal%20of%20analytical%20methods%20in%20chemistry&rft.au=Schou-Pedersen,%20Anne%20Marie%20Voigt&rft.date=2018-01-01&rft.volume=2018&rft.issue=2018&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.issn=2090-8865&rft.eissn=2090-8873&rft_id=info:doi/10.1155/2018/6148515&rft_dat=%3Cgale_doaj_%3EA587142191%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c576t-a09f59c5966e2388418a94be85514186b98a4e5dec2e0d234cd03ecb76bf559b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2000985241&rft_id=info:pmid/29484214&rft_galeid=A587142191&rfr_iscdi=true