Molecular Visualization of Early‐Stage Acute Kidney Injury with a DNA Framework Nanodevice

DNA nanomachines with artificial intelligence have attracted great interest, which may open a new era of precision medicine. However, their in vivo behavior, including early diagnosis and therapeutic effect are limited by their targeting efficiency. Here, a tetrahedral DNA framework (TDF)‐based nano...

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Bibliographic Details
Published in:Advanced science 2022-07, Vol.9 (20), p.e2105947-n/a
Main Authors: Ding, Fei, Zhang, Shuangye, Liu, Suyu, Feng, Jing, Li, Jiang, Li, Qian, Ge, Zhilei, Zuo, Xiaolei, Fan, Chunhai, Xia, Qiang
Format: Article
Language:English
Subjects:
acute kidney injury
Acute Kidney Injury - diagnosis
Acute Kidney Injury - metabolism
Artificial Intelligence
Biomarkers
DNA - metabolism
DNA nanodevice
early diagnosis
Humans
Kidney - metabolism
kidney injury molecule‐1
Kidneys
Microscopy
Peptides
Physiology
tetrahedral DNA framework
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Summary:DNA nanomachines with artificial intelligence have attracted great interest, which may open a new era of precision medicine. However, their in vivo behavior, including early diagnosis and therapeutic effect are limited by their targeting efficiency. Here, a tetrahedral DNA framework (TDF)‐based nanodevice for in vivo near‐infrared (NIR) diagnosis of early‐stage AKI is developed. This nanodevice comprises three functional modules: a size‐tunable TDF nanostructure as kidney‐targeting vehicle, a binding module for the biomarker kidney injury molecule‐1 (Kim‐1), and a NIR signaling module. The cooperation of these modules allows the nanodevice to be selectively accumulated in injured kidney tissues with high Kim‐1 level, generating strong NIR fluorescence; whereas the nanodevice with the proper size can be rapidly cleared in healthy kidneys to minimize the background. By using this nanodevice, the early diagnosis of AKI onset is demonstrated at least 6 h ahead of Kim‐1 urinalysis, or 12 h ahead of blood detection. It is envisioned that this TDF‐based nanodevice may have implications for the early diagnosis of AKI and other kidney diseases. A tetrahedral DNA framework‐based nanodevice integrating three functionalities allows real‐time tracking of the dynamic variation of kidney injury molecule‐1 in high contrast manner, and can be used as imaging agent and urinalysis tracers to non‐invasively diagnose the onset of acute kidney injury earlier than that can be detected by the conventional in vitro assays.
ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202105947