Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis

• Published in: Mediators of Inflammation 2012-01, Vol.2012 (2012), p.1003-1012
• Main Authors: Li, Zhiliang, Fu, Qiang, Wang, Ming, Zhang, Weiwei, Cao, Longxing, Yan, Wen, Zhao, Yiqiao, Jin, Wen, Zhang, Ting
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Coronary Artery Disease - blood; Coronary Stenosis - blood; Coronary Stenosis - pathology; Dendritic Cells - metabolism; Female; Humans; Interleukin-17 - blood; Lipoproteins, LDL - blood; Male; Middle Aged
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/2012/506283

Coronary artery disease (CAD) is an immune-mediated chronic inflammatory disease mainly caused by atherosclerosis. The aims of this study were to investigate the role of interleukin-27 (IL-27) in patients with CAD and the severity of coronary artery lesions, which was evaluated by Gensini score and to investigate the biosynthesis of IL-27 and oxidized low-density lipoprotein (ox-LDL) in vitro using monocyte-derived dendritic cells (DCs). To this aim, plasma levels of IL-27, ox-LDL, and Gensini score were analyzed in patients with CAD (n=136) and normal subjects (controls, n=29). IL-27 concentration of the supernatant and the mRNA expression levels of p28 and ebi3, subunits of IL-27, from cultured immature DCs incubated with different concentrations of ox-LDL for 24 h were also analyzed. We found that circulating IL-27 levels were significantly elevated in patients with CAD than in controls (P