Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats

• Published in: Scientific reports 2018-02, Vol.8 (1), p.2105-11, Article 2105
• Main Authors: Liang, Ching-Chung, Shaw, Sheng-Wen Steven, Huang, Yung-Hsin, Lin, Yi-Hao, Lee, Tsong-Hai
• Format: Article
• Language: English
• Subjects: 13; 13/100; 64; 692/4025/1334; 692/617/375/430; 82; 82/51; Acetylcholine receptors (muscarinic); Amniotic fluid; Amniotic Fluid - chemistry; Animals; Bladder; Body weight; Cells, Cultured; Contraction; Deoxyguanosine; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - physiopathology; Disease Models, Animal; Female; Humanities and Social Sciences; Immunoreactivity; multidisciplinary; Nerve growth factor; Rats; Rats, Sprague-Dawley; Rodents; Science; Science (multidisciplinary); Stem Cell Transplantation; Stem cells; Stem Cells - cytology; Streptozocin; Transplantation; Urinary bladder; Urinary Bladder - physiopathology; Urologic Diseases - etiology; Urologic Diseases - therapy
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-20512-z

To examine the effects of human amniotic fluid stem cells (hAFSCs) transplantation on bladder function and molecular changes in diabetic rats, 60 female Sprague-Dawley rats were used for study. Three groups were assigned including sham control rats, streptozotocin (STZ, 60 mg/kg)-induced diabetic rats and STZ-induced diabetic rats plus bladder hAFSCs transplantation. Compared to controls, diabetic rats had decreased body weight but increased bladder weight. Cystometries showed non-voiding contraction, residual volume, voided volume and intercontraction interval increased significantly in diabetic rats at week 4 and 12 after DM induction, but improved after hAFSCs transplantation. The immunoreactivities and mRNAs of nerve growth factor (NGF) decreased significantly in diabetic bladder at week 4 and 12 after DM induction, but recovered after hAFSCs transplantation. The immunoreactivities and mRNAs of M2 and M3 muscarinic receptor increased significantly in diabetic bladder at week 4 after DM induction but recovered after hAFSCs transplantation. The immunoreactivity of 8-hydroxy-20-deoxyguanosine increased significantly in diabetic bladder at week 4 and 12 after DM induction but reduced after hAFSCs transplantation. The present study showed bladder dysfunction in STZ-induced diabetic rats could be improved by hAFSCs transplantation into bladder, which may be related to the recovery of bladder NGF and muscarinic receptors.