Effects of myeloperoxidase on inflammatory responses with hypoxia in Citrobacter rodentium‐infectious mice

Purpose Myeloperoxidase (MPO) has been identified as a mediator in various inflammatory diseases. Bacterial infection of the intestine and hypoxia can both lead to inflammatory responses, but the role of MPO in these phenomena remains unclear. Methods By building the MPO‐/‐ mice, we evaluated releva...

Full description

Saved in:
Bibliographic Details
Published in:Immunity, Inflammation and Disease Inflammation and Disease, 2024-02, Vol.12 (2), p.e1157-n/a
Main Authors: Gao, Xiang, Zhang, Yu, Zhu, Qinfang, Han, Ying, Jia, Ruhan, Zhang, Wei
Format: Article
Language:English
Subjects:
Animals
Antibodies
Bacterial Infections
Biotechnology
Chemokines
Citrobacter rodentium
colitis
Colon
Hyperplasia
Hypoxia
Infections
Inflammatory bowel disease
inflammatory responses
Laboratory animals
Metabolism, Inborn Errors
Mice
MPO
Neutrophils
Peroxidase
Spleen
Tumor Necrosis Factor-alpha
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose Myeloperoxidase (MPO) has been identified as a mediator in various inflammatory diseases. Bacterial infection of the intestine and hypoxia can both lead to inflammatory responses, but the role of MPO in these phenomena remains unclear. Methods By building the MPO‐/‐ mice, we evaluated relevant inflammatory factors and tissue damage in mice with intestinal Citrobacter rodentium infection and hypoxia. The body weight and excreted microorganisms were monitored. Intestinal tissues were collected 7 days after bacterial infection under hypoxia to undergo haematoxylin‐eosin staining and assess the degree of pathological damage. ELISA assays were performed to quantify the serum levels of TNF‐α, IFN‐γ, IL‐6, and IL‐1β inflammatory cytokines. PCR, WB, and IF assays were conducted to determine the expression of chemokines MCP1, MIP2, and KC in the colon and spleen. Results The C. rodentium infection and hypoxia caused weight loss, intestinal colitis, and splenic inflammatory cells active proliferation in wild‐type mice. MPO deficiency alleviated this phenomenon. MPO‐/‐ mice also displayed a significant decline in bacteria clearing ability. The level of TNF‐α in the serum and spleen was both lower in MPO‐/‐ hypoxia C. rodentium‐infected mice than that in wild‐type mice. The chemokines expression levels of MIP2, KC, and MCP1 in the spleen and colon of each bacterial infected group were significantly increased (p 
ISSN:2050-4527
2050-4527
DOI:10.1002/iid3.1157