Histone Modification Marks Strongly Regulate CDH1 Promoter in Prostospheres as A Model of Prostate Cancer Stem Like Cells

Cadherin-1 ( ) plays an important role in the metastasis, while expression of this protein is under control of epigenetic changes on its gene promoter. Therefore we evaluated both DNA methylation (DNAmet) and histone modification marks of in prostate cancer stem like cells (PCSLCs). In this experime...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2019-07, Vol.21 (2), p.124-134
Main Authors: Shokraii, Fatemeh, Moharrami, Maryam, Motamed, Nasrin, Shahhoseini, Maryam, Totonchi, Mehdi, Ezzatizadeh, Vahid, Firouzi, Javad, Khosravani, Pardis, Ebrahimi, Marzieh
Format: Article
Language:English
Subjects:
Cadherins
Cancer Stem Cells
CD44 antigen
CDH1
Cell adhesion & migration
Cell culture
Cell surface
CpG islands
Deoxyribonucleic acid
DNA
DNA methylation
E-cadherin
Epidermal growth factor
Epigenetics
Gene expression
Histone Modification
Histones
Metastases
Metastasis
Methylation
Original
Penicillin
Polymerase chain reaction
Prostate Cancer
Spheres
Stem cells
Surface markers
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Summary:Cadherin-1 ( ) plays an important role in the metastasis, while expression of this protein is under control of epigenetic changes on its gene promoter. Therefore we evaluated both DNA methylation (DNAmet) and histone modification marks of in prostate cancer stem like cells (PCSLCs). In this experimental study, we isolated PCSLCs using cell surface marker and prostaspheroid formation, respectively. The cells isolated from both methods were characterized and then the levels of H3K4me2, H3K27me3, H3K9me2/3 and H3K9ac as well as DNAmet were assessed in promoter of the isolated cells. The CD44 CD49 cells were not validated as PCSLCs. However, prostaspheres overexpressed stemness related genes and had higher ability of invasion potential, associated with reduction in expression. Epigenetic status analysis showed that promoter was hypo-methylated. Histone modifications of H3K9ac and H3K4me3 were significantly reduced, in parallel with an increased level of H3K27me3. Our results suggest that slight decrease of DNAmet of the CpG island in promoter does not significantly contribute to the change of expression. Therefore, histone modifications are responsible in repressing in PCSLCs.
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2019.5702