Genome-wide identification and expression analysis of NF-Y gene family in tobacco (Nicotiana tabacum L.)

Nuclear factor Y (NF-Y) gene family is an important transcription factor composed of three subfamilies of NF-YA , NF-YB and NF-YC , which is involved in plant growth, development and stress response. In this study, 63 tobacco NF-Y genes ( NtNF-Ys ) were identified in Nicotiana tabacum L., including...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2024-03, Vol.14 (1), p.5257-5257, Article 5257
Main Authors: Tian, Yue, Song, Kangkang, Li, Bin, Song, Yanru, Zhang, Xiaohua, Li, Haozhen, Yang, Long
Format: Article
Language:English
Subjects:
631/208/191/2018
631/208/199
631/208/726
631/449/2661
Drought
Genes
Genomes
Humanities and Social Sciences
Light effects
multidisciplinary
NF-Y gene
Nicotiana tabacum
Phylogeny
Plant growth
Protein structure
Science
Science (multidisciplinary)
Tobacco
Tomatoes
Transcriptomes
Y gene
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nuclear factor Y (NF-Y) gene family is an important transcription factor composed of three subfamilies of NF-YA , NF-YB and NF-YC , which is involved in plant growth, development and stress response. In this study, 63 tobacco NF-Y genes ( NtNF-Ys ) were identified in Nicotiana tabacum L., including 17 NtNF-YAs , 30 NtNF-YBs and 16 NtNF-YCs . Phylogenetic analysis revealed ten pairs of orthologues from tomato and tobacco and 25 pairs of paralogues from tobacco. The gene structure of NtNF-YAs exhibited similarities, whereas the gene structure of NtNF-YBs and NtNF-YCs displayed significant differences. The NtNF-Ys of the same subfamily exhibited a consistent distribution of motifs and protein 3D structure. The protein interaction network revealed that NtNF-YC12 and NtNF-YC5 exhibited the highest connectivity. Many cis-acting elements related to light, stress and hormone response were found in the promoter of NtNF-Ys . Transcriptome analysis showed that more than half of the NtNF-Y genes were expressed in all tissues, and NtNF-YB9/B14/B15/B16/B17/B29 were specifically expressed in roots. A total of 15, 12, 5, and 6 NtNF-Y genes were found to respond to cold, drought, salt, and alkali stresses, respectively. The results of this study will lay a foundation for further study of NF-Y genes in tobacco and other Solanaceae plants.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-55799-8