Identification of a Profile of Neutrophil-Derived Granule Proteins in the Surface of Gold Nanoparticles after Their Interaction with Human Breast Cancer Sera

It is well known that the interaction of a nanomaterial with a biological fluid leads to the formation of a protein corona (PC) surrounding the nanomaterial. Using standard blood analyses, alterations in protein patterns are difficult to detect. PC acts as a “nano-concentrator” of serum proteins wit...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2020-06, Vol.10 (6), p.1223
Main Authors: Chantada-Vázquez, María del Pilar, García-Vence, María, Vázquez-Estévez, Sergio, Bravo, Susana B., Núñez, Cristina
Format: Article
Language:English
Subjects:
Biomarkers
Breast cancer
breast cancer (BC)
Cancer therapies
Chemotherapy
Concentrators
Disease
Gold
gold nanoparticles (AuNPs)
Granular materials
Immune system
Innate immunity
Irritable bowel syndrome
mass spectrometry (MS)
Medical prognosis
Methods
Nanomaterials
Nanoparticles
neutrophils
Peptides
protein biomarkers
Proteins
Proteomes
Proteomics
Serum proteins
Sodium
surface
Transmission electron microscopy
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Summary:It is well known that the interaction of a nanomaterial with a biological fluid leads to the formation of a protein corona (PC) surrounding the nanomaterial. Using standard blood analyses, alterations in protein patterns are difficult to detect. PC acts as a “nano-concentrator” of serum proteins with affinity for nanoparticles’ surface. Consequently, characterization of PC could allow detection of otherwise undetectable changes in protein concentration at an early stage of a disease, such as breast cancer (BC). Here, we employed gold nanoparticles (AuNPsdiameter: 10.02 ± 0.91 nm) as an enrichment platform to analyze the human serum proteome of BC patients (n = 42) and healthy controls (n = 42). Importantly, the analysis of the PC formed around AuNPs after their interaction with serum samples of BC patients showed a profile of proteins that could differentiate breast cancer patients from healthy controls. These proteins developed a significant role in the immune and/or innate immune system, some of them being neutrophil-derived granule proteins. The analysis of the PC also revealed serum proteome alterations at the subtype level.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano10061223