Modulation of immunological responses by aqueous extract of Datura stramonium L. seeds on cyclophosphamide-induced immunosuppression in Wistar rats

• Published in: BMC immunology 2022-10, Vol.23 (1), p.50-19, Article 50
• Main Authors: Joshua, Parker Elijah, Yahaya, Junaidu, Ekpo, Daniel Emmanuel, Ogidigo, Joyce Oloaigbe, Odiba, Arome Solomon, Asomadu, Rita Onyekachukwu, Oka, Samson Ayodeji, Adeniyi, Olasupo Stephen
• Format: Article
• Language: English
• Subjects: 3-Carene; Acids; Animals; Antigens; Antioxidants; Antioxidants - pharmacology; Asthma; Body weight; Catalase; Catechin; Chemical properties; Chlorogenic Acid; Complications and side effects; Conjunctivitis; Cyclophosphamide; Cytokines; Datura stramonium; Datura stramonium L; Drug dosages; Enzymes; Epilepsy; Filariasis; Flavonoids; Gallic acid; Gallic Acid - pharmacology; Glutathione Peroxidase; Health aspects; Immune response; Immune system; Immunoglobulin A; Immunoglobulin G; Immunoglobulins; Immunological research; Immunomodulation; Immunostimulation; Immunosuppression; Immunosuppression Therapy; Jimson weed; Levamisole; Limonene; Lipoproteins; Lipoproteins, HDL; Luteolin; Materia medica, Vegetable; Medicinal plants; Phytochemicals; Plant extracts; Plant Extracts - pharmacology; Quercetin; Quinine; Rats; Rats, Wistar; Rheumatoid arthritis; Rodents; Saponins; Seeds; Superoxide Dismutase; Tannins; Tumor necrosis factor-TNF; Vanillic Acid; Vitamins; Water
• ISSN: 1471-2172; 1471-2172
• DOI: 10.1186/s12865-022-00519-y

Datura stramonium L. (Solanaceae) is used traditionally in west Africa to treat asthma, epilepsy, rheumatoid arthritis, filariasis microbial infections and conjunctivitis. This study investigated the immunomodulatory effects of aqueous seed extract of D. stramonium L. (ASEDS) on Wistar rats. Thirty Wistar albino rats (180-200 g) were randomized into 6 groups (n = 5). Group 1 received distilled water only. Rats in groups 2-6 were pretreated with 10 mg/kg body weight (b.w.) Cyclophosphamide orally for 27-days to induce immunosuppression. Thereafter, they received treatment orally for 28 days as follows: Group 2 (distilled water), group 3 (5 mg/kg b.w. Levamisole), groups 4-6 (60, 90 and 120 mg/kg b.w. ASEDS, respectively). HPLC was used to determine major compounds in ASEDS. The effects of ASEDS on immune cells, immunoglobulins A, G and M levels, lipoproteins, and antioxidant status of rats were evaluated. ASEDS indicated high content of Acutumine, Quinine, Catechin, Chlorogenic acid, Gallic acid, Quercetin, Vanillic acid, Luteolin, Formosanin C, Saponin, Cyanidin, Tannic acid, 3-Carene, Limonene and α-terpineol. Cyclophosphamide triggered significant (p