ACSS3 in brown fat drives propionate catabolism and its deficiency leads to autophagy and systemic metabolic dysfunction

• Published in: Clinical and translational medicine 2022-02, Vol.12 (2), p.e665-n/a
• Main Authors: Jia, Zhihao, Chen, Xiyue, Chen, Jingjuan, Zhang, Lijia, Oprescu, Stephanie N., Luo, Nanjian, Xiong, Yan, Yue, Feng, Kuang, Shihuan
• Format: Article
• Language: English
• Subjects: acyl‐CoA synthetase; Adipocytes; Adipocytes, Brown - drug effects; Adipocytes, Brown - metabolism; Adipocytes, White - drug effects; Adipocytes, White - metabolism; adipose tissue; Adipose Tissue, Brown - drug effects; Adipose Tissue, Brown - growth & development; Animals; Autophagy; Body fat; brown adipocytes; Cell growth; Clinical medicine; Coenzyme A Ligases - adverse effects; Coenzyme A Ligases - pharmacology; Disease Models, Animal; Enzymes; Fatty acids; Food; Gene expression; Glucose; hydroxychloroquine; Insulin resistance; Lipids; Metabolic disorders; Mice; Mice, Knockout - metabolism; Obesity; Propionates - metabolism; Propionates - pharmacology; short‐chain fatty acid
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1002/ctm2.665

Propionate is a gut microbial metabolite that has been reported to have controversial effects on metabolic health. Here we show that propionate is activated by acyl‐CoA synthetase short‐chain family member 3 (ACSS3), located on the mitochondrial inner membrane in brown adipocytes. Knockout of Acss3 gene (Acss3–/–) in mice reduces brown adipose tissue (BAT) mass but increases white adipose tissue (WAT) mass, leading to glucose intolerance and insulin resistance that are exacerbated by high‐fat diet (HFD). Intriguingly, Acss3–/– or HFD feeding significantly elevates propionate levels in BAT and serum, and propionate supplementation induces autophagy in cultured brown and white adipocytes. The elevated levels of propionate in Acss3–/– mice similarly drive adipocyte autophagy, and pharmacological inhibition of autophagy using hydroxychloroquine ameliorates obesity, hepatic steatosis and insulin resistance of the Acss3–/– mice. These results establish ACSS3 as the key enzyme for propionate metabolism and demonstrate that accumulation of propionate promotes obesity and Type 2 diabetes through triggering adipocyte autophagy. Acss3 is highly expressed in BAT and Acss3 knockout inhibits BAT adipogenesis, leading to smaller brown adipose tissue in vivo. Loss of Acss3 causes elevated circulating propionate that promotes lipid accumulation and autophagy in adipose tissue. Pharmacological inhibition of autophagy using hydroxychloroquine ameliorates obesity, hepatic steatosis and insulin resistance of the Acss3–/– mice.