Extraction Condition Optimization, Quantitative Analysis, and Anti-AD Bioactivity Evaluation of Acorn Polyphenols from Quercus variabilis, Quercus aliena, and Quercus dentata

• Published in: International journal of molecular sciences 2024-10, Vol.25 (19), p.10536
• Main Authors: Du, Jianing, Han, Zhengkun, Ran, Longyi, Zhang, Taiyu, Li, Junru, Li, Huiying
• Format: Article
• Language: English
• Subjects: Acids; acorn polyphenol; Acorns; Alzheimer's disease; Analysis; Animals; Antioxidants; Disease; Ellagic Acid - chemistry; Ellagic Acid - pharmacology; endogenous phenolics; Gallic Acid - chemistry; Gallic Acid - pharmacology; Genetic engineering; LC-MC/MS; Mice; Mice, Transgenic; Multifactorial traits; Oak; Oxidative stress; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Polyphenols - chemistry; Polyphenols - pharmacology; Properties; Quantitative analysis; quercetin; Quercus - chemistry; Retention; Tissues; Transgenic animals
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms251910536

In the present study, and acorn kernels were taken as the research objects, and the free polyphenols and bound polyphenols in acorn kernels were extracted using improved ultrasound-assisted ethanolic and alkaline extraction methods, after which the contents of gallic acid, quercetin, azelaic acid, ellagic acid, and ferulic acid were quantified by LC-MC/MS. The results demonstrated that and acorns were suitable as raw materials to extract ellagic acid, the contents of ferulic acid and bound gallic acid in them were different, and acorns were more suitable for the research of gallic acid, but not for azelaic acid. Results on APP/PS1 transgenic mice verified that five polyphenols significantly suppressed the progression of AD. This study provides a theoretical basis for the drug development of acorn polyphenols.