Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR

Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infe...

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Bibliographic Details
Published in:Medical mycology case reports 2023-03, Vol.39, p.34-37
Main Authors: Spanamberg, Andréia, Ravazzolo, Ana Paula, Araujo, Ricardo, Franceschi, Natália, Ferreiro, Laerte
Format: Article
Language:English
Subjects:
Case Report
Cattle
Dermatophytes
Hair
Ringworm
Trichophyton verrucosum
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Summary:Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by real‐time PCR and melting‐point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.
ISSN:2211-7539
2211-7539
DOI:10.1016/j.mmcr.2023.01.002