Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran

Some mosquito species which belong to the complex are primary vectors for West Nile virus, Sindbis, , and many arboviruses. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene of that is inherited, is one of the important threats for the efficacy of pyrethroids insec...

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Bibliographic Details
Published in:Iranian journal of public health 2019-06, Vol.48 (6), p.1091-1098
Main Authors: Zeidabadinezhad, Reza, Vatandoost, Hassan, Abai, Mohammad Reza, Dinparast Djadid, Navid, Raz, Abbasali, Sedaghat, Mohammad Mahdi, Oshaghi, Mohamad Ali, Raeisi, Ahmad, Adibi, Neda
Format: Article
Language:English
Subjects:
Culex pipiens
Deltamethrin
Disease resistance
Genotypes
Genotyping
Insecticide resistance
Insecticides
Insects
Knockdown resistance
Larvae
Mosquitoes
Mutation
Nucleotides
Original
Point mutation
Population studies
Pyrethroids
Real time
Sodium channels (voltage-gated)
Species
Target detection
Vectors
Viruses
Wastewater
Wastewater pollution
West Nile virus
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Summary:Some mosquito species which belong to the complex are primary vectors for West Nile virus, Sindbis, , and many arboviruses. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene of that is inherited, is one of the important threats for the efficacy of pyrethroids insecticides. Knockdown resistance (kdr) mutation, L1014F, is a well-defined mechanism of resistance to pyrethroids and DDT in many insect species. The aim of study was to determine the mechanisms of Insecticide resistance in this species. Specimens of , the major vector of West Nile virus, were obtained in Tehran, Iran by collecting larvae from polluted wastewater in Qarchak of Tehran. In 2016 Insecticide susceptibility tests were performed according to WHO methods with deltamethrin 0.05%. We focused on determination of this point mutation in the VGSC gene of by Real-time PCR. Our results revealed high levels of resistance to deltamethrin 0.05%. The lethal times i.e. LT and LT for deltamethrin were 2.1530 and 8.5117 h respectively. The result of Real-time PCR confirmed the presence of resistant genotype in all the members of tested population. This study is the first report on kdr genotyping of from Tehran and our results on the VGSC gene in position L1014F confirmed the TTA to TTT nucleotide change. This finding will provide a clue for management of insecticide resistance in mosquito which are vectors of arboviruses and decision for replacement of novel approach for vector control.
ISSN:2251-6085
2251-6093
DOI:10.18502/ijph.v48i6.2916