The impact of different levels of wheat diets on hepatic oxidative stress, immune response, and lipid metabolism in Tibetan sheep (Ovis aries)

• Published in: BMC veterinary research 2024-01, Vol.20 (1), p.26-26, Article 26
• Main Authors: Ma, Boyan, Raza, Sayed Haidar Abbas, Pant, Sameer D, Gao, Zhanhong, Zhang, Fengshuo, Wang, Zhiyou, Hou, Shengzhen, Alkhateeb, Mariam Abdulaziz, Al Abdulmonem, Waleed, Alharbi, Yousef Mesfer, Aljohani, Abdullah S M, Gui, Linsheng
• Format: Article
• Language: English
• Subjects: Analysis; Animal husbandry; Antioxidants; Body weight; Cell activation; Cell differentiation; Correlation analysis; Diet; Diseases; Feeding trials; Feeds; Gene expression; Genes; Genetic aspects; Genomes; Glutathione peroxidase; Growth; GSEA; Health aspects; Helper cells; Hepatocytes; Immune response; Immunoglobulin A; Immunoglobulin M; Immunoglobulins; Leukocyte migration; Lipid metabolism; Liver; Livestock; Lymphocytes T; Metabolism; Metabolites; Nutrient deficiency; Oxidative stress; Proteins; RNA-Seq; Sheep; Signal transduction; Software; Tibetan lamb; Transcriptomics; Wheat diet
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-023-03874-z

Compared with corn, wheat contains higher crude protein, amino acids concentration. However, wheat contains a mass of anti-nutritional factors, resulting in increased of the digesta viscosity and impaired the intestinal function in ruminant. This study aimed to investigate the effects of substitution of different amounts of wheat for corn on hepatic metabolism in the Tibetan lamb. Ninety Tibetan lambs (Body weight = 12.37 ± 0.92 kg) were randomly assigned to three groups: 0% wheat diet (Control), 10% wheat diet (Low group), and 15% wheat diet (High group). The feeding trial lasted for 130 d, including a 10 d adaption period. Hepatic gene expression profiling was performed via RNA sequencing after the conclusion of the feeding trials. Results showed that greater level of glutathione peroxidase levels in L group compared with those of the C and H groups (P