Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research

The fertilised chick egg and particularly its chorioallantoic membrane (CAM) have drawn continuing interest in biomedicine and bioengineering fields, especially for research on vascular study, cancer, drug screening and development, cell factors, stem cells, etc. This literature review systemically...

Full description

Saved in:
Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-02, Vol.10 (2), p.463
Main Authors: Chen, Lei, Wang, Shuncong, Feng, Yuanbo, Zhang, Jinyong, Du, Yuqing, Zhang, Jiang, Ongeval, Chantal Van, Ni, Yicheng, Li, Yue
Format: Article
Language:English
Subjects:
Angiogenesis
Animal welfare
Biomedical research
Blood
chick embryo
Chorioallantoic membrane
chorioallantoic membrane (CAM)
Drug screening
Eggs
Embryos
imaging techniques
Literature reviews
Medical research
Membranes
Pharmaceutical industry
Pharmacokinetics
Physiology
preclinical pharmacological research
Review
Stem cells
Toxicology
vascular disrupting agents (VDAs)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The fertilised chick egg and particularly its chorioallantoic membrane (CAM) have drawn continuing interest in biomedicine and bioengineering fields, especially for research on vascular study, cancer, drug screening and development, cell factors, stem cells, etc. This literature review systemically introduces the CAM's structural evolution, functions, vascular features and the circulation system, and cell regulatory factors. It also presents the major and updated applications of the CAM in assays for pharmacokinetics and biodistribution, drug efficacy and toxicology testing/screening in preclinical pharmacological research. The time course of CAM applications for different assays and their advantages and limitations are summarised. Among these applications, two aspects are emphasised: (1) potential utility of the CAM for preclinical studies on vascular-disrupting agents (VDAs), promising for anti-cancer vascular-targeted therapy, and (2) modern imaging technologies, including modalities and their applications for real-time visualisation, monitoring and evaluation of the changes in CAM vasculature as well as the interactions occurring after introducing the tested medical, pharmaceutical and biological agents into the system. The aim of this article is to help those working in the biomedical field to familiarise themselves with the chick embryo CAM as an alternative platform and to utilise it to design and optimise experimental settings for their specific research topics.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells10020463