Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout

• Published in: Toxins 2023-01, Vol.15 (1), p.48
• Main Authors: Gruber-Dorninger, Christiane, Killinger, Manuela, Höbartner-Gußl, Andreas, Rosen, Roy, Doupovec, Barbara, Aleschko, Markus, Schwartz-Zimmermann, Heidi, Greitbauer, Oliver, Marković, Zoran, Stanković, Marko, Schöndorfer, Karin, Vukmirovic, Djuro, Wein, Silvia, Schatzmayr, Dian
• Format: Article
• Language: English
• Subjects: Animal feed; Animal Feed - analysis; Animal species; Animals; Biomarkers; Chickens; Chickens - metabolism; Clay; Contaminants; Degradation; enzyme; Enzymes; Estrogenic activity; Estrogens; Feces; feed additive; Feed additives; Feeds; gastrointestinal; Gastrointestinal system; Gastrointestinal tract; Gastrointestinal Tract - metabolism; Hogs; hydrolase; Immune system; Inactivation; Metabolites; Mycotoxins; Oncorhynchus mykiss; Oncorhynchus mykiss - metabolism; Poultry; Swine; Trout; Xenoestrogens; Zearalenone; Zearalenone - metabolism
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins15010048

The estrogenic mycotoxin zearalenone (ZEN) is a common contaminant of animal feed. Effective strategies for the inactivation of ZEN in feed are required. The ZEN-degrading enzyme zearalenone hydrolase ZenA (EC 3.1.1.-, commercial name ZEN , BIOMIN Holding GmbH, Getzersdorf, Austria) converts ZEN to hydrolyzed ZEN (HZEN), thereby enabling a strong reduction in estrogenicity. In this study, we investigated the efficacy of ZenA added to feed to degrade ZEN in the gastrointestinal tract of three monogastric animal species, i.e., pigs, chickens, and rainbow trout. For each species, groups of animals received (i) feed contaminated with ZEN (chickens: 400 µg/kg, pigs: 200 µg/kg, rainbow trout: 2000 µg/kg), (ii) feed contaminated with ZEN and supplemented with ZenA, or (iii) uncontaminated feed. To investigate the fate of dietary ZEN in the gastrointestinal tract in the presence and absence of ZenA, concentrations of ZEN and ZEN metabolites were analyzed in digesta of chickens and rainbow trout and in feces of pigs. Upon ZenA administration, concentrations of ZEN were significantly decreased and concentrations of the degradation product HZEN were significantly increased in digesta/feces of each investigated animal species, indicating degradation of ZEN by ZenA in the gastrointestinal tract. Moreover, upon addition of ZenA to the diet, the concentration of the highly estrogenic ZEN metabolite α-ZEL was significantly reduced in feces of pigs. In conclusion, ZenA was effective in degrading ZEN to HZEN in the gastrointestinal tract of chickens, pigs, and rainbow trout, and counteracted formation of α-ZEL in pigs. Therefore, ZenA could find application as a ZEN-degrading feed additive for these animal species.