Non-destructive collection and metabarcoding of arthropod environmental DNA remained on a terrestrial plant

Reliable survey of arthropods is a crucial for their conservation, community ecology, and pest control on terrestrial plants. However, efficient and comprehensive surveys are hindered by challenges in collecting arthropods and identifying especially small species. To address this issue, we developed...

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Bibliographic Details
Published in:Scientific reports 2023-05, Vol.13 (1), p.7125-7125, Article 7125
Main Authors: Yoneya, Kinuyo, Ushio, Masayuki, Miki, Takeshi
Format: Article
Language:English
Subjects:
704/158
704/158/670
Animals
Arthropoda
Arthropods
Arthropods - genetics
Biodiversity
Community ecology
Cytochrome-c oxidase
DNA - genetics
DNA Barcoding, Taxonomic - methods
DNA, Environmental - genetics
Drinking water
Environmental DNA
Humanities and Social Sciences
Introduced species
multidisciplinary
Pest control
Plant extracts
Plants - genetics
Science
Science (multidisciplinary)
Surveys
Water
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Summary:Reliable survey of arthropods is a crucial for their conservation, community ecology, and pest control on terrestrial plants. However, efficient and comprehensive surveys are hindered by challenges in collecting arthropods and identifying especially small species. To address this issue, we developed a non-destructive environmental DNA (eDNA) collection method termed “plant flow collection” to apply eDNA metabarcoding to terrestrial arthropods. This involves spraying distilled or tap water, or using rainfall, which eventually flows over the surface of the plant, and is collected in a container that is set at the plant base. DNA is extracted from collected water and a DNA barcode region of cytochrome c oxidase subunit I (COI) gene is amplified and sequenced using a high-throughput Illumina Miseq platform. We identified more than 64 taxonomic groups of arthropods at the family level, of which 7 were visually observed or artificially introduced species, whereas the other 57 groups of arthropods, including 22 species, were not observed in the visual survey. These results show that the developed method is possible to detect the arthropod eDNA remained on plants although our sample size was small and the sequence size was unevenly distributed among the three water types tested.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-32862-4