Isolation, Culture and Characterization of Human Synovium-Derived Mesenchymal Stem Cells

• Published in: Cell journal (Yakhteh) 2009-07, Vol.11 (2), p.160-167
• Main Authors: Kadivar, Mehdi, Darvish, Maryam, Moghadam, Masood Salehi
• Format: Article
• Language: English
• Subjects: Knee; Mesenchymal Stem Cells; Synovium; Tissue
• ISSN: 2228-5806; 2228-5814

Objective: It has been demonstrated that mesenchymal stem cells (MSCs), which are isolatedfrom various tissues, have different potential in differentiation and proliferation. Forthis reason it is necessary to isolate these cells from various sources in order to use them inclinical settings. The present study has been done to investigate the possibility of isolation,culture and characterization of human synovium-derived mesenchymal stem cells.Materials and Methods: Samples (200-300 mg) were provided from synovium tissue ofpatients who had knee surgery. Obtained samples were homogenized, enzymatically mincedwith collagenase D and passed through 70 μm nylon filters; separated cells were then cultured.Isolated cells were identified by morphological observations, differentiation tests, flowcytometry, immunocytochemistery studies and RT-PCR.Results: The isolated cells in this study showed fibroblast-like morphology and have a highproliferation capacity. In flow cytometry and immunocytochemical studies, they were positivefor CD73 and CD105 antigens. RT-PCR analysis and specific staining for differentiated cellstowards osteogenic and adipogenic lineages, showed that isolated cells were potent in differentiationinto the mentioned lineages.Conclusion: These results suggest that synovium tissue, which is discarded in most kneeoperations, can be used for cell therapy and tissue engineering protocols as an enrichmentsource of potent mesenchymal stem cells.