Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in Caenorhabditis elegans

• Published in: Antioxidants 2023-01, Vol.12 (1), p.125
• Main Authors: Zhang, Sai-Ya, Qin, Zi-Chen, Sun, Yi-Yang, Chen, Yu-Si, Chen, Wen-Bo, Wang, Hong-Gang, An, Di, Sun, Dan, Liu, Yan-Qiang
• Format: Article
• Language: English
• Subjects: Aging; anti-heat stress; antioxidant; Antioxidants; Caenorhabditis elegans; Cytotoxicity; Dietary restrictions; Experiments; Flavonoids; Genistein; Heat shock factors; Heat shock proteins; Heat stress; Hydrogen peroxide; Insulin; Kinases; Life expectancy; Life span; Localization; Lymphocytes T; MAP kinase; Mitochondria; Mutants; Nematodes; Oxidative stress; Physiology; Reactive oxygen species; Sodium; Survival; Temperature effects; Transcription factors
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox12010125

To determine the anti-heat stress and antioxidant effects of genistein and the underlying mechanisms, lipofuscin, reactive oxygen species (ROS), and survival under stress were first detected in ); then the localization and quantification of the fluorescent protein was determined by detecting the fluorescently labeled protein mutant strain; in addition, the aging-related mRNAs were detected by applying real-time fluorescent quantitative PCR in . The results indicate that genistein substantially extended the lifespan of under oxidative stress and heat conditions; and remarkably reduced the accumulation of lipofuscin in under hydrogen peroxide (H O ) and 35 °C stress conditions; in addition, it reduced the generation of ROS caused by H O and upregulated the expression of , , , , , , , and , whereas it downregulated the expression of and in similarly, it upregulated the expression of , , , , , , , , , and , whereas it downregulated the expression of , , , and in at 35 °C; moreover, it increased the accumulation of HSP-16.2 and SKN-1 proteins in nematodes under 35 °C and H O conditions; however, it failed to prolong the survival time in the deleted mutant MQ130 nematodes under 35 °C and H O conditions. These results suggest that genistein promote anti-heat stress and antioxidant effects in via insulin/-insulin-like growth factor signaling (IIS), heat shock protein (HSP), mitogen-activated protein kinase (MAPK), dietary restriction (DR), and mitochondrial pathways.